Summary
Preparations of ultrathin frozen sections of the retina and choroid in rat were studied by the new technique of Tokuyasu (1973). All membrane structures were observable in ultrathin frozen sections, so that this method is likely to be most valuable for the morphologic study of membranes. However, there are some disadvantages to the use of this technique in that identification of some intracellular organelles such as ribosomes and glycogen is difficult. A fibrous dense network was observed in the interphotoreceptor spaces, the intermicrovilli spaces of the pigment epithelium, and it appeared in the space between the photoreceptor and the microvilli. The network almost disappeared after treatment with hyaluronidase for 30 min, suggesting that it may consist of acid mucopolysaccharide.
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References
Bernhard, W., Nancy, M.T.: Coupes à congelation ultrafines de tissue inclus dans la gelatine. J. Microscopie. 3, 579–588 (1964)
Bernhard, W., Viron, A.: Improved techniques for the preparation of ultrathin frozen sections. J. Cell Biol. 49, 731–746 (1971)
Christensen, A.K.: Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver. J. Cell Biol. 51, 772–804 (1971)
Feeney, L.: The interphotoreceptor space, I. Postnatal ontogeny in mice and rats. Develop. Biol. 32, 101–114 (1973)
Feeney, L.: The interphotoreceptor space, II. Histochemistry of the matrix. Develop. Biol. 32, 115–128 (1973)
Fernandez-Moran, H., Orville, A.D.: Electron microscopy of ultrathin frozen sections of pollen grains. Science. 116, 465–467 (1952)
Fine, B.S.: Limiting membranes of the sensory retina and pigment epithelium, an electron microscopic study. Arch. Ophthal. 66, 847–860 (1961)
Fine, B.S., Zimmerman, L.E.: Observation on the rod and cone layer of the human retina, a light and electron microscopic study. Invest. Ophthalmol. 2, 446–459 (1963)
Fitton-Jackson, S.: The Cell, 387 New York: Academic Press 1966
Hodson, S., Marshall, J.: Ultracryotomy: a technique for cutting ultrathin sections of unfixed frozen biological tissue for electron microscopy. J. Micro. 91, 105–117 (1970)
Hansson, H.A.: Scanning electron microscopy of the rat retina. Z. Zellforsch. 107, 23–44 (1970)
Koda, N.: The Preparation of Ultrathin Frozen Sections of the Retina in Rat, I. Acta Soc. Ophthalm. Jap. 77, 1973–1981 (1973)
Koda, N.: The Preparation of Ultrathin Frozen Sections of the Retina in Rat, II. Acta Soc. Ophthalm. Jap. 78, 324–331 (1974)
Koda, N.: The Preparation of Ultrathin Frozen Sections of the Retina in Rat, III. Acta Soc. Ophthalm. Jap. 78, 686–695 (1974)
Koda, N., Mizuno, K.: Preparation of ultrathin frozen sections of the retina. Jap. J. Ophthal. 18, 226–232 (1974)
Kuhlman, W., Viron, A.: Cross-linked albumin as supporting matrix ultrathin cryomicrotomy. J. Ultrastruct. Res. 41, 385–394 (1972)
Luft, J.H.: Electron Microscopy. Ed., by Ueda, R., 2, p. 65. Tokyo: Maruzen Co. 1966
Tokuyasu, K.T.: A technique for ultracryotomy of all suspensions and tissues. J. Cell Biol. 57, 551–565 (1973)
Zimmerman, L.E., Easthan, A.B.: Acid mucopolysaccharide in the retinal pigment epithelium and visual cell layer of the developing mouse eye. Am. J. Ophthalmol. 47, 488–499 (1959)
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Maekawa, N., Mizuno, K. Cryoultramicroscopy of the retina and choroid in rat. Albrecht von Graefes Arch. Klin. Ophthalmol. 200, 113–121 (1976). https://doi.org/10.1007/BF00414360
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DOI: https://doi.org/10.1007/BF00414360