Abstract
Rhodopseudomonas sulfoviridis is unable to grow with sulfate as sole sulfur source. Radioactively labelled sulfate is not incorporated into the cells. Growth only occurs in the presence of reduced sulfur compounds, such as sulfide, thiosulfate, elemental sulfur and cysteine. ATP sulfurylase, adenylylsulfate kinase, O-acetylserine sulfhydrylase and cysteine desulfhydrase are present. Adenylylsulfate sulfotransferase and thiosulfonate reductase are lacking. The enzymes of the sulfate-activating system are not derepressed by O-acetylserine.
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Abbreviations
- APS:
-
Adenosine 5′-phosphosulfate
- PAPS:
-
3′-phosphoadenosine 5′-phosphosulfate
References
Chambers LA, Trudinger PA (1971) Cysteine and S-sulfocysteine biosynthesis in bacteria. Arch Mikrobiol 77:165–184
Cooper BP (1980) Der enzymatische Mechanismus der Sulfataktivierung bei Rhodospirillaceae. Doctoral Thesis, University of Bonn, FRG
Hansen TA, Veldkamp H (1973) Rhodopseudomonas sulfidophila, nov. spec., a new species of the purple nonsulfur bacteria. Arch Mikrobiol 92:45–58
Hansen TA (1974) Sulfide als electrondonor voor Rhodospirillaceae. Doctoral Thesis, University of Groningen, Holland
Imhoff JF (1980) Aspekte des assimilatorischen Schwefelstoffwechsels in Rhodospirillaceae. Doctoral Thesis, University of Bonn, FRG
Imhoff JF, Then J, Hashwa F, Trüper HG (1981) Sulfate assimilation in Rhodopseudomonas globiformis. Arch Microbiol 130:234–237
Imhoff JF, Trüper HG (1977) Ectothiorhodospira halochloris, sp. nov., a new extremely halophilic phototrophic bacteria containing bacteriochlorophyll b. Arch Microbiol 114:115–121
Keppen OI, Gorlenko VM (1975) A new species of purple budding bacteria containing bacteriochlorophyll b. Mikrobiologiya 44:258–263
Kumagai H, Sejima S, Choi YJ, Tanaka H, Yamada H (1975) Crystallization and properties of cysteine desulfhydrase from Aerobacter aerogenes. FEBS Lett 52:304–307
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
Pachmayr F (1966) Vorkommen und Bestimmung von Schwefelverbindungen in Mineralwasser. Doctoral Thesis, University of München, FRG
Pfennig N (1974) Rhodopseudomonas globiformis, sp. n., a new species of the Rhodospirillaceae. Arch Microbiol 100:197–206
Roy AB, Trudinger PA (1970) The biochemistry of inorganic compounds of sulfur. Cambridge University Press: 43–44
Schmidt A (1972) On the mechanism of photosynthetic sulfate reduction. An APS-sulfotransferase from Chlorella. Arch Mikrobiol 84:77–86
Schmidt A (1973) Sulfate reduction in a protein-bound intermediate by a thiosulfonate reductase. Arch Mikrobiol 93:29–52
Siegel LM (1975) Biochemistry of the sulfur cycle. In: Greenberg DM (ed) Metabolic pathways, VII. Metabolism of sulfur compounds. Academic, New York, pp 217–286
Stickland LH (1951) The determination of small quantities of bacteria by means of the biuret reaction. J Gen Microbiol 5:698–703
Wilson LG, Bandurski RS (1958) Enzymatic reactions involving sulfate, sulfite, selenate and molybdate. J Biol Chem 233:975–981
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Neutzling, O., Trüper, H.G. Assimilatory sulfur metabolism in Rhodopseudomonas sulfoviridis . Arch. Microbiol. 133, 145–148 (1982). https://doi.org/10.1007/BF00413529
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DOI: https://doi.org/10.1007/BF00413529