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The influence of hopanoids on growth of Mycoplasma mycoides

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Abstract

Hopane glycolipid, isolated from Bacillus acidocaldarius, was studied in its ability to support growth from Mycoplasma mycoides var. capri as substitute for sterols. All attempts to culture Mycoplasma on hopane glycolipid failed. When added together with cholesterol, the effect of HGL was a reduction in growth rate.

The hopanoid diplopterol moderately supported growth of Mycoplasma. This effect was strikingly enhanced in diplopterol adapted cultures. Application of diplopterol via dipalmitoyl-phosphatidylcholine liposomes resulted in an improved growth rate. These results suggested that diplopterol can fullfill sterol function in Mycoplasma membranes.

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Abbreviations

HGL:

Hopane glycolipid 1-(O-β-N-acylglucosaminyl)-2,3,4-tetrahydroxypentane-29-hopane

diplopterol:

22-hydroxy-hopane

DPPC:

dipalmitoyl-phosphatidylcholine

PC:

phosphatidylcholine

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Authors and Affiliations

  1. Lehrbereich Mikrobiologie I, Institut für Biologie II der Universität Tübingen, Auf der Morgenstelle 28, D-7400, Tübingen, Federal Republic of Germany

    Elmar Kannenberg & Karl Poralla

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  1. Elmar Kannenberg
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  2. Karl Poralla
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Kannenberg, E., Poralla, K. The influence of hopanoids on growth of Mycoplasma mycoides . Arch. Microbiol. 133, 100–102 (1982). https://doi.org/10.1007/BF00413519

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  • DOI: https://doi.org/10.1007/BF00413519

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