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A detailed restriction endonuclease cleavage map of rat liver mitochondrial DNA

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Summary

A restriction endonuclease cleavage map of rat liver mitochondrial DNA is presented for the following enzymes: Xba I, Bgl II, Hae II, Bam HI, Hpa I, Hha I, Bcl I, Hind II, Hind III, Eco RI, Hpa II, Hae III, and Sau 3A. It was derived from complete and partial digestions with these enzymes, double digestions, and redigestions of defined fragments obtained with one enzyme with other restriction enzymes. By the use of these and further enzymes (Taq YI, Alu I) the mitochondrial DNA (ca. 15.6 Kb) can be dissected into a large number of defined fragments.

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Abbreviations

mtDNA:

mitochondrial DNA

bp or Kbp:

base pairs or kilo base pairs

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität, Goethestrasse 33, D-8000, München 2, Germany

    Horst Feldmann & Rüdiger Großkopf

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  1. Horst Feldmann
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  2. Rüdiger Großkopf
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Feldmann, H., Großkopf, R. A detailed restriction endonuclease cleavage map of rat liver mitochondrial DNA. Curr Genet 1, 13–19 (1979). https://doi.org/10.1007/BF00413303

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