Abstract
The photoreceptor cell endings of goldfish retinas were examined using ultrathin and freeze-fracture techniques. The synaptic vesicles could be seen lying closely packed in rows on each side of the synaptic ribbon, and the vesicles nearest to the end of the ribbon could be seen to be in direct contact with the presynaptic membrane. The openings of the synaptic vesicles to the synaptic cleft could also be observed at the presynaptic membrane nearest to the end of the ribbon, that is, 100 nm distant from the center of the apex. In the freeze-fractured replicas of the presynaptic membrane, the band-shaped membrane particle aggregation was seen at the apex and a row of the crater-like or circular protuberances were observed at each side of the apex. These structures were considered to be changes of the presynaptic membrane organizations according to the synaptic vesicle exocytosis. It may be possible that the synaptic vesicle exocytosis in the photoreceptor cells occurs at the sites of the presynaptic membrane nearest to the apical end of the synaptic ribbon, and that the exocytotic sites form a line on each side of the apex.
Zusammenfassung
Die Fotorezeptorzellendigungen in der Goldfischretina wurden mit Ultradünnschnitten und unter Anwendung der Gefrierätztechnik untersucht.
Die synaptischen Vesikel finden sich dicht gepackt in Reihen auf jeder Seite der synaptischen Bänder; die Vesikel am Ende der synaptischen Bänder besitzen dabei einen direkten Kontakt mit der präsynaptischen Membran. Öffnungen zwischen synaptischen Vesikeln und der präsynaptischen Membran konnten auch nahe der Enden der synaptischen Bänder beobachtet werden: dies entspricht etwa einer Distanz von ca. 100 nm vom sog. “Apex’. In Gefrierbrüchen der präsynaptischen Membran kann eine bandartige Aggregation von membrangebundenen Partikeln im Bereich des ‘Apex’ nachgewiesen werden; zusätzlich findet sich eine Reihe von kraterartigen bzw. zirkulären Protuberanzen auf jeder Seite des ‘Apex’. Diese Strukturen werden als Veränderungen in der Organisation der präsynaptischen Membran im Sinne der Exocytose von synaptischen Vesikeln angesehen.
Es ist möglich, daß die Exocytose von synaptischen Vesikeln in Fotorezeptorzellen in Bereichen der synaptischen Membran stattfindet, die nahe dem aphikalen Ende der synaptischen Bänder liegen und daß die Bereiche, in denen die Exocytose stattfindet, Linien auf jeder Seite des ‘Apex’ bilden.
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Matsumura, M., Okinami, S. & Ohkuma, M. Synaptic vesicle exocytosis in goldfish photoreceptor cells. Albrecht von Graefes Arch. Klin. Ophthalmol. 215, 159–170 (1981). https://doi.org/10.1007/BF00413147
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DOI: https://doi.org/10.1007/BF00413147