Summary
We studied contact photosensitivity (CPS) to tetrachlorosalicylanilide (TCSA) in BALB/cJ mice with various doses of TCSA and UVA at sensitization and challenge. From these studies we recommend the following protocol: sensitization on days 0 and 1 with 50 μl of 1% TCSA followed by 3 J/cm2 of UVA, and challenge on day 7 with 10 μl of 3% TCSA followed by 6 J/cm2 of UVA. This gave an ear swelling response of 27.4±0.6×10-3 (mean±standard error). We also demonstrated that there is reciprocity between volume and concentration of TCSA at sensitization but not between TCSA and UVA doses at sensitization.
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References
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This study was supported by the Medical Research Council of Canada and the Ontario Ministry of Health. This work was presented in part at the 1988 annual meeting of the Society for Investigative Dermatology, Washington, D.C.
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Brown, W.R., Furukawa, R.D., Shivji, G.M. et al. Optimization of tetrachlorosalicylanilide and ultraviolet A doses a sensitization and challenge for contact photosensitivity in the mouse. Arch Dermatol Res 281, 351–354 (1989). https://doi.org/10.1007/BF00412981
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DOI: https://doi.org/10.1007/BF00412981