Skip to main content
Log in

Decreased DNA repair activity in sunburn cells

A possible pathogenetic factor of the epidermal sunburn reaction

  • Original Works
  • Published:
Archives of Dermatological Research Aims and scope Submit manuscript

Summary

The pathogenesis of the formation of sunburn cells is unknown. Based on autoradiographic methods the unscheduled DNA repair synthesis of UV-induced thymin dimers was investigated in vivo in sunburn cells and in irradiated but histologically normal stratum spinosum cells. The results show a significant lower number of sparsly labeled cells in the sunburn cell-population (13.2±2.5; mean) when compared to the population of normal stratum spinosum cells (57.8±7.5; mean). These data indicate that the population of those epidermal cells, which become manifest as sunburn cells 24 h after UV exposure exhibit a reduced DNA repair of UV induced thymine dimers immediately after UV irradiation. Nuclear factors thus seem to play at least some role in the origin of sunburn cells.

Zusammenfassung

Die Pathogenese der Entstehung von Sonnenbrandzellen ist unbekannt. Die nicht schematisierte (unscheduled) DNA-Synthese von UV-induzierten Thymindimeren wurde in Sonnenbrandzellen sowie in UV-bestrahlten, aber histologisch unauffälligen Stratum spinosum-Zellen autoradiographisch in vivo untersucht. Es fand sich die Zahl locker markierter Zellen innerhalb der Sonnenbrandzellen-Population (13,2±2,5; Mittelwert) gegenüber der Zahl locker markierter Zellen in der Population normaler Stratum spinosum-Zellen (57,8±7,5; Mittelwert) signifikant vermindert.

Die Resultate deuten darauf hin, daß die Population jener Epidermalzellen, die 24 h nach UV-Bestrahlung zu Sonnenbrandzellen werden, kurz nach der UV-Exposition eine verminderte DNS-Reparatur von Thymindimeren aufweist.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Cripps, D. J., Ramsay, C. A., Carter, J., Boutwell, R.: Effect of monochromatic UV radiation on DNA synthesis with in vivo and in vitro autoradiography. J. Invest. Dermatol. 58, 312–314 (1972)

    Google Scholar 

  2. Epstein, W. L., Fukuyama, K., Epstein, J. H.: Early effects of ultraviolet light on DNA synthesis in human skin in vivo. Arch. Dermatol. 100, 84–89 (1969)

    Google Scholar 

  3. Hönigsmann, H., Wolff, K., Konrad, K.: Epidermal lysosomes and ultraviolet light. J. Invest. Dermatol. 63, 337–342 (1974)

    Google Scholar 

  4. Johnson, B. E., Mandell, G., Daniel, S. F., Jr.: Melanin and cellular reactions to ultraviolet radiation. Nature New Biol. 235, 147–149 (1972)

    Google Scholar 

  5. Magnus, I. A.: Reaction of normal skin to UVR and to visible light. In: Dermatological photobiology, Magnus, I.A., pp. 117–146. Oxford, London, Edinburgh, Melburne: Blackwell Sci. Publ. 1976

    Google Scholar 

  6. Nix, T. E. Jr.: Ultraviolet induced changes in epidermis. Ultrastructure of normal and abnormalskin. Zelickson, A. S. (ed.), pp. 304–319. Philadelphia: Lea & Febiger 1967

    Google Scholar 

  7. Wolff, K., Gschnait, F., Hönigsmann, H., Konrad, K., Parrish, J. A., Fitzpatrick, T. B.: Phototesting and dosimetry for photochemotherapy. Br. J. Dermatol. 96, 1–10 (1977)

    Google Scholar 

  8. Woodcock, A., Magnus, I. A.: The sunburn cell in mouse skin: preliminary quantitative studies on its production. Br. J. Dermatol. 95, 459–468 (1976)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brenner, W., Gschnait, F. Decreased DNA repair activity in sunburn cells. Arch. Dermatol. Res. 266, 11–16 (1979). https://doi.org/10.1007/BF00412857

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00412857

Key words

Schlüsselwörter

Navigation