Summary
Competetive protein binding technique represents a new very sensitive, specific and rapid method for determination of minute amounts of endogenous steroids. It was used for determination of corticosteroids in the rabbit primary and secondary aqueous humors, as well as in the plasma. Three different sets of aqueous humor samples were analyzed, namely primary aqueous from the left eye, secondary aqueous from the same eye, and primary aqueous from the right eye, both latter withdrawn after an interval of three hours: values of 7.76±4.33, 4.61±2.22, and 4.37±2 73 ng per ml, respectively, were obtained. Plasmatic values, at corresponding time intervals, were nearly ten-times higher, i. e. 5.6+1.65, and 5.9+0.98 μg per 100 ml, respectively. Parallel flurometric determination of plasmatic levels of corticosterone and cortisol was in good agreement, showing 4–5 times prevalence of the corticosterone over cortisol.
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Berardinis, E. de, Bonavolontá, G.: Ricerche sperimentali sul passagio e sulla concentrazione del cortisone nell'umore acqueo e nel vitreo. Rass. ital. Ottal. 22, 157–167 (1953).
Castrén, J. A., Raitta, C., Laamanen, A.: Über das Eindringen eines DepotCorticosteroids in die Vorderkammer und den Glaskörper, und die Verweildauer der Steroids im Auge. Acta Ophthal. (Kbh.) 42, 680–684 (1964).
Chouraqui, J., Weniger, J.-P.: Identification des corticosteroides secretés par les surrenales embryonnaires de veau, lapin et souris cultivées in vitro. Acta endocr. (Kbh.) 65, 650–662 (1970).
Fazekas, I. G., Fazekas, A. T.: Papierchromatographischer Nachweis der Corticosteroidfunktion in Kaninchenorganen und-geweben. Endokrinologie 50, 130–138 (1966).
Fevold, H. R.: The pathways of corticosteroid biosynthesis by homogenates of adrenal tissue from rabbits stimulated with adrenocorticotropin. Biochemistry 8, 3433–3439 (1969).
Glaucoma. Transactions of the Second Conference, p. 9, December 3, 4, and 5, 1956, Princeton, N. J., edit. by F. W. Newell. New York: Josiah Macy Jr. Foundation 1957.
Green, H., Kroman, H., Leopold, I. H.: Corticosteroids in the aqueous humor of the rabbit. Arch. Ophthal. 54, 853–857 (1955a).
—, Kroman, H. S., Leopold, I. H.: Corticosteroids in the aqueous humor of the rabbit eye. Further studies with paper chromatography. Amer. J. Ophthal. 44, (Pt. 2), 91–94 (1957).
—, Leopold, I. H.: Investigation of corticosteroids in the aqueous humor of normal animal eyes. Amer. J. Ophthal. 38, (Pt. 2), 101–106 (1954).
—, Sawyer, J. L., Leopold, I. H.: Investigation of corticosteroids in the aqueous humor of normal rabbit eyes. II. Further observations. Amer. J. Ophthal. 39, 871–873 (1955b).
Havener, W. H.: Ocular pharmacology, 2nd ed., p. 274. St. Louis: The C. V. Mosby Company 1970.
Kass, E. H., Hechter, O., Macchi, I. A., Mou, T. W.: Changes in patterns of secretion of corticosteroids in rabbits after prolonged treatment with ACTH. Proc. Soc. exp. Biol. (N.Y.) 85, 583–587 (1954).
Kronfeld, P. C.: Protein content of aqueous humor in man. Amer. J. Ophthal. 24, 1121 (1941).
Krumm, A. A., Glenn, R. E.: Adrenal steroid secretion in rabbits following prolonged ACTH administration. Proc. Soc. exp. Biol. (N.Y.) 118, 255–258 (1965).
Leopold, I. H., Barnert, A. H.: Steroids in ophthalmology. Adv. Ophthal. 18, 1–58 (1967).
—, Maylath, F. C.: Intraocular penetration of cortisone and its effectiveness against experimental corneal burns. Amer. J. Ophthal. 35, 1125–1134 (1952a).
——: Corticoid determinations of the aqueous humor after repeated paracentesis. Amer. J. Ophthal. 35, 932–933 (1952b).
Murphy, B. E. P.: Application of the property of protein binding to the assay of minute quantities of hormones and other substances. Nature (Lond.) 201, 679–682 (1964).
—: Some studies of the protein-binding of steroids and their application to the routine micro and ultramicro measurements of various steroids in body fluids by competetive protein-binding radioassay. J. clin. Endocr. 27, 973 (1967).
Obenberger, J., Praus, R.: Changes in the protein content and composition of the rabbit aqueous humor, in samples withdrawn from both eyes. Čs. Oftal. 20, 333–336 (1964).
Pegg, P. J., Keane, P. M.: The simultaneous estimation of plasma cortisol and transcortin binding characteristics by a competetive protein binding technique. Steroids 14, 705–715 (1969).
Peterson, R. E., Pierce, R. E.: The metabolism of corticosterone in man. J. clin. Invest. 39, 741–757 (1960).
—, Wyngaarden, J. B., Guerra, S. L., Brodie, B. B., Bunim, J. J.: The physiological disposition and metabolic fate of hydrocortisone in man. J. clin. Invest. 34, 1779–1794 (1955).
Stárka, L., Hampl, R., Obenberger, J.: Corticosterone in aqueous humor of the rabbit eye. J. Steroid Biochem. (In press.)
Trichtel, F., Papapanos, G.: Vergleichende Corticosteroidbestimmungen im normalen Kammerwasser verschiedener Säugetiere. Albrecht v. Graefes Arch. klin. exp. Ophthal. 161, 325–328 (1959).
Vies, J. van der: Individual determination of cortisol and corticosterone in a single small sample of peripheral blood. Acta endocr. (Kbh.) 38, 399 (1961).
Younessian, S.: Glaucome et cataracte après corticothérapie par voie générale et locale. Adv. Ophthal. 23, 74–152 (1970).
Yudaev, N. A., Afinogenova, S. A.: Changes in the function of adrenal glands in rabbits under the effect of cortisone and sodium salicylate. Probl. Endokr. Gormonter. 6, 19–25 (1960).
—, Morozova, M. S.: The activity of 21 and 11β-hydroxylases in the adrenal glands of rabbits subjected to repeated ACTH administration. Probl. Endokr. Gormonter. 11, 81–87 (1965).
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Obenberger, J., Stárka, L. & Hampl, R. Quantitative determination of endogenous corticosteroids in the rabbit plasma and aqueous humor. Albrecht von Graefes Arch. Klin. Ophthalmol. 183, 203–209 (1971). https://doi.org/10.1007/BF00412825
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DOI: https://doi.org/10.1007/BF00412825