Summary
The acute in vitro action of ACTH and corticosterone individually and in combination were determined in the B-16 melanoma grown in vivo. ACTH elevated levels 10-fold while tyrosinase activity generally was depressed. Corticosterone depressed cAMP levels and tyrosinase activity. ACTH in the presence of corticosterone produced a coincident peak in tyrosinase activity and cAMP levels followed by a depression of enzymatic activity. The results demonstrate that cAMP is not the sole modulator of tyrosinase activity and that ACTH, corticosterone and cAMP interact in the regulation of B-16 melanoma tyrosinase activity.
Zusammenfassung
ACTH erhöht den cAMP-Spiegel zehnfach, während die Tyrosinaseaktivität gesenkt wurde. Corticosteron senkt den cAMP-Spiegel und auch die Tyrosinaseaktivität. Bei gleichzeitiger Gabe von Corticosteron führt ACTH zu einer koinzidenten Erhöhung der Tyrosinaseaktivität und der cAMP-Spiegel, der eine Aktivitätssenkung des Enzyms folgt. Die Untersuchungen zeigen, daß cAMP nicht der einzige Modulator der Tyrosinaseaktivität ist und das ACTH, Corticosteron und cAMP in die Regulation der Tyrosinase-Aktivität des B-16-Melanoms zusammenspielen.
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Abramowitz, J., Chavin, W. Effect of ACTH and corticosterone on melanogenesis and cyclic nucleotide levels in the B-16 melanoma. Arch Dermatol Res 264, 293–298 (1979). https://doi.org/10.1007/BF00412656
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DOI: https://doi.org/10.1007/BF00412656