Summary
Sera of patients with various malignancies are known to contain DNA-binding proteins (DBP) which are not present in sera of normal individuals. In this paper sera of patients with malignant melanoma (MM) were examined as to whether characteristic DBP are present, too.
DBP are isolated by DNA-affinity chromatography and represent 0.5–0.9% of all serum proteins. After separation of the DBP by SDS slab gel electrophoresis no typical DBP is detectable in sera of MM-patients. However, quantitative differences are found in sera of patients in the clinical stages I–III and/or tumor level 3–5:
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1.
All 9 sera of patients who had clinical signs of MM contain more DBP with molecular weight (mw) of 20,000–24,000 dalton than control sera. However, these DBP are only increased in 30% of the 22 sera from MM-patients who had clinical signs for 13–73 months after tumor excision.
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2.
All sera of the 10 MM-patients of whom sera were drawn twice after tumor excision at an interval of 7–46 months without clinical signs, showed a reduction of DBP with mw 30,000, 68,000, and 165,000.
Zusammenfassung
Im Serum von Patienten mit malignen Tumoren wurden DNA-affine Proteine (DBP) nachgewiesen, die nicht im Serum Gesunder vorkommen. Hiervon ausgehend wurden Seren von Patienten mit Malignem Melanom (MM) auf charakteristische DBP untersucht.
Die DBP werden durch DNA-Affinitätschromatographie isoliert und stellen 0.5–0.9% aller Serumproteine dar. Nach Auftrennung der DBP im SDS-Plattengel können keine für MM-Seren charakteristische DBP gefunden werden. Quantitative Unterschiede sind jedoch in Seren von MM-Patienten im klinischen Stadium I–III bzw. Tumor-Level 3–5 zu finden:
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1.
In allen 9 Seren von Patienten mit klinischen Erscheinungen eines MM treten DBP mit Molekulargewicht (MG) von 20–24000 vermehrt auf. Diese DBP sind jedoch nur noch in 30% der 22 Seren von MM-Patienten vermehrt, die nach Tumorexcision 13–73 Monate klinisch erscheinungsfrei waren.
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2.
Die Seren aller 10 MM-Patienten, denen nach der Tumor-Excision in erscheinungsfreien Intervallen von 7–46 Monaten zweimal Blut entnommen wurden, zeigen eine Verminderung der DPB mit MG 30000, 68000 und 165000 im längeren erscheinungsfreien Intervall.
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Abbreviations
- DBP:
-
DNA-binding proteins
- MM:
-
malignant melanoma; mw—molecular weight of ... dlatons
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Dedicated to Prof. Dr. Stüttgen on his 60th birthday
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Reimer, G., Maibach, M. & Leonhardi, G. DNA-binding proteins in the sera of patients with malignant melanoma. Arch Dermatol Res 264, 265–273 (1979). https://doi.org/10.1007/BF00412653
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DOI: https://doi.org/10.1007/BF00412653