Summary
We examined granulocytes or polymorphonuclear leukocytes (PMN) in an HLA B8+ patient with palmoplantar pustulosis (PPP). Controls included another patient with PPP, however, lacking this antigen and a healthy, HLA B8+ person. Chemiluminescence (CL) served to monitor the respiratory burst in PMN comparing as stimuli zymosan, opsonized zymosan, phorbol myristate acetate, as well as aggregated immunoglobulin (aggIg), the latter as Fc-receptor (FcR) stimulus. FcR density on PMN was determined using 125I-IgG and expressed in the form of Scatchard plots. The effects of serum on the aggIg-induced CL were also measured. We found both control individuals to respond to stimulation by aggIg as a function of a dose-dependent increase of CL. By contrast, the HLA B8+ patient with PPP failed to respond to aggIg; only the highest concentration of aggIg induced marginal CL. Conversely, stimulation by the other agents was similar in all three individuals. The patient with the functional FcR defect expressed 2.5 times more FcR/PMN than the controls. No difference emerged in comparing autologous serum with a reference normal serum on the aggIg-induced CL, ruling out saturation by serum factors alone to be a cause for the defect. In remission, the functional FcR was absent. Our results suggest a defect of signal transduction in PMN from numerically enhanced FcR to the cytosol in the patient with PPP.
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References
Baker H, Wilkinson DS (1979) Persistent palmoplantar pustulosis. In: Rook A, Wilkinson DS, Ebling FJG (eds) Textbook of dermatology. Blackwell, Oxford London, pp 1365–1366
Ennen J, Ernst M, Flad HD (1986) Fc-receptor mediated and non-specific phagocytic stimulation of lymphokineactivated monocytes as detected by chemiluminescence. J Bioluminesc Chemiluminesc 1:96
Frank MM, Hamburger MI, Lawley TJ, Kimberly RP, Plotz PH (1979) Defective reticuloendothelial system Fc-receptor function in systemic lupus erythematosus. N Engl J Med 300:518–523
Fries LF, Mullins WW, Cho KR, Plotz PH, Frank MM (1984) Monocyte receptors for the Fc portion of IgG are increased in systemic lupus erythematosus. J Immunol 132:695–700
Fülöp T, Fóris G, Wórum I, Leövey A (1985) Age-dependent alterations of Fc gamma receptor-mediated effector functions of human PMN. Clin Exp Immunol 61:425–432
Geerdink JPM, Troost PW, Schalwijk J, Joosten LAB, Mier PD (1985) The “metabolic burst” in polymorphonuclear leukocytes from patients with quiescent psoriasis. Br J Dermatol 112:387–392
Guyre PM, Crabtree GR, Bodwell JE, Munck A (1981) MLC-conditioned media stimulate an increase in Fc-receptors on human macrophages. J Immunol 126:666–668
Hamburger MI, Moutsopoulos HM, Lawley TJ, Frank MM (1979) Sjögren's syndrome: a defect in reticuloendothelial system Fc-receptor-specific clearance. Ann Int Med 91: 534–538
Iwatsuki K, Imaizumi S, Tsugiki M, Yamada M, Tagami H (1985) Alterations of surface receptors on intralesional neutrophils in pustular psoriasis and palmo-plantar pustulosis. Br J Dermatol 112:53–56
Kelton JG, Singer J, Rodger C, Gauldie J, Horsewood P, Dent P (1985) The concentration of IgG in serum is a major determinant of Fc-dependent reticuloendothelial function. Blood 66:490–495
Lawley TJ, Hall RP, Fauci AS, Katz SI, Hamburger MI, Frank MM (1981) Defective Fc-receptor functions associated with the HLA-B8/DRw3 haplotype. N Engl J Med 304:185–192
Lehrer RI, Cohen L (1981) Receptor-mediated regulation of superoxide production in human neutrophils stimulated by phorbol myristate acetate. J Clin Invest 68:1314–1320
Lundin A, Hakansson L, Hällgren R, Michaelsson G, Venge P (1983) Studies on the phagocytic activity of the granulocytes in psoriasis and palmoplantar pustulosis. Br J Dermatol 109:539–547
Müller-Quernheim T, Schopf RE, Benes P, Rubin R, Schulz V (1984) Selective unresponsiveness of alveolar macrophages in pulmonary alveolar proteinosis. In: Kricka LJ, Stanley PE, Thorpe GHG, Whitehead (eds) Analytical applications of bioluminescence and chemiluminescence. Academic Press, London Orlando, pp 307–310
Neppert J, Clemens M, Mueller-Eckhardt C (1986) Immune phagocytosis inhibition by commercial immunoglobulins. Blut 52:67–72
Patrone F, Dallegri F, Bonvini E, Frumento G, Nocera A, Ferrani M, Sacchetti C (1983) Modulation of neutrophil Fc- and C3b-receptors. Inflammation 7:155–168
Potter M (1981) Immunoglobulins M, G, and A. In: Oppenheim JJ, Rosenstreich DL, Potter M (eds) Cellular functions in immunity and inflammation. Elsevier, New York Amsterdam, pp 229–258
Scatchard G (1949) The attraction of proteins for small molecules and ions. Ann NY Acad Sci 51:660–672
Schopf RE, Mattar J, Meyenburg W, Scheiner O, Hammann KP, Lemmel E-M (1984) Measurement of the respiratory burst in human monocytes and polymorphonuclear leukocytes by nitro blue tetrazolium reduction and chemiluminescence. J Immunol Method 67:109–117
Schwartz BD (1984) The human major histocompatibility HLA complex. In: Stites DP, Stobo JD, Fudenberg HH, Wells JV (eds) Basic and clinical immunology, 5th edn. Lange, Los Altos, CA, pp 55–68
Uracz W, Pituch-Noworolska A, Popiela T, Zembala M (1982) The Fc-receptors of normal and cancer patients' monocytes. Immunobiology 163:450–459
Ward JM, Corbett MF, Hanna MJ (1976) A double-blind trial of clomocycline in the treatment of persistent palmoplantar pustulosis. Br J Dermatol 95:317–322
Zachariae H, Overgaard Petersen H, Kissmeyer Nielsen F, Lamm L (1977) HLA antigens in pustular psoriasis. Dermatologica 154:73–77
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Schopf, R.E., Rehder, M., Benes, P. et al. Impaired function of numerically augmented Fc-receptors on granulocytes in a HLA B8+ patient with palmoplantar pustulosis. Arch Dermatol Res 279, 444–448 (1987). https://doi.org/10.1007/BF00412589
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DOI: https://doi.org/10.1007/BF00412589