Summary
In order to study the β-adrenergic receptorcyclic AMP system in the human skin, we have developed a monoclonal antibody against the receptor binding site in two fusion steps. We produced an anti-alprenolol monoclonal antibody using a hybridoma technique after the immunization of a mouse with alprenolol-BSA conjugate. This antibody was then used to immunize mice, whose splenocytes were used for the second fusion. Colonies were screened by enzyme linked immunosorbent assay (ELISA) using turkey erythrocyte membrane as an antigen. One of the positive colonies was grown in mice to obtain ascites fluids. This antibody showed the ability to compete with [125I]-iodocyanopindolol for the binding to β-adrenergic receptors of turkey erythrocytes. The binding of the antibody to the turkey erythrocytes was prevented by the existence of the anti-alprenolol antibody and also L-alprenolol. This antibody showed a prominent inhibition of the adrenaline-stimulated adenylate cyclase activity of the turkey erythrocyte membrane. The data indicate that the anti-idiotypic monoclonal antibody against alprenolol binds to the β-adrenergic receptors and acts like an antagonist.
We further studied the localization of the β-adrenergic receptors with this antibody in the normal human skin as well as in the psoriatic skin using immunohistochemical technique. A prominent decrease of the β-receptors was observed in the psoriatic epidermis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adachi K, Halprin KM, Takeda J, Nemoto O, Aoyagi T, Iizuka H, Yoshikawa K, Levine V (1982) Epidermal surface receptors which link pharmacological mediators to the adenylate cyclase system. Br J Dermatol 107:111–118
Cleveland WL, Wasserman NH, Sarangarajan R, Penn AS, Erlanger BF (1983) Monoclonal antibodies to the acetylcholine receptor by a normally functioning auto-antiidiotypic mechanism. Nature 305:56–67
Fraser CM, Venter JC (1980) Monoclonal antibodies to β-adrenergic receptors: use in purification and molecular characterization of β-receptors. Proc Natl Acad Sci USA 77:7034–7038
Gazith J, Reichert U (1982) High affinity membrane receptors in cultured human keratinocytes. I. The β-adrenergic receptors. Br J Dermatol 107 [Suppl]:125–133
Guillet JG, Kaveri SV, Durieu O, Delavier C, Hoebeke J, Strosberg AD (1983) β-Adrenergic agonist activity of a monoclonal anti-idiotypic antibody. Proc Natl Acad Sci USA:1781–1784
Hoebeke J, Vanquelin G, STrosberg AD (1978) The production and characterization of antibodies against β-adrenergic antagonist. Biochem Pharmacol 27:1527–1532
Homcy CJ, Rockson SG, Haber E (1982) An antiidiotypic antibody that recognizes the β-adrenergic receptor. J Clin Invest 69:1147–1154
Iizuka H, Adachi K, Halprin KM, Levine V (1978) Cyclic AMP accumulation in psoriatic skin: differential responses to histamine, AMP, and epinephrine by the uninvolved and involved epidermis. J Invest Dermatol 70:250–253
Itami S, Tsutsui M, Kino J, Koizumi H, Katayama H, Halprin KM, Adachi K (1986) Monoclonal antibodies to the β-adrenergic receptor: modulation of catecholaminesensitive adenylate cyclase by the antibody. Arch Dermatol Res 278:377–381
King WJ, Greene GL (1984) Monoclonal antibodies localize oestrogen receptor in the nuclei of target cells. Nature 307:745–747
Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497
Roth RA, Cassell DJ, Wong KY, Maddux BA, Goldfine ID (1982) Monoclonal antibodies to the human insulin receptor block insulin binding and inhibit insulin action. Proc Natl Acad Sci USA 79:7312–7316
Salomon Y (1979) Adenylate cyclase assay. Adv Cyclic Nucleotide Protein Phosphorylation Res 10:35–55
Schreiber AB, Couraud PO, Andre C, Vray B, Strosberg AD (1980) Anti-alprenolol anti-idiotypic antibodies bind to β-adrenergic receptors and modulate catecholaminesensitive adenylate cyclase. Proc Natl Acad Sci USA 77:7385–7389
Wrenn S, Haber E (1979) An antibody specific for the propranolol binding site of cardiac muscle. J Biol Chem 254:6577–6589
Yoshikawa K, Adachi K, Halprin KM, Levine V (1975) On the lack of response to catecholamine stimulation by the adenylate cyclase system in proriatic lesions. Br J Dermatol 92:619–624
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Itami, S., Kino, J., Halprin, K.M. et al. Immunohistochemical study of β-adrenergic receptors in the psoriatic epidermis using an anti-alprenolol anti-idiotypic antibody. Arch Dermatol Res 279, 439–443 (1987). https://doi.org/10.1007/BF00412588
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00412588