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The electrophoretic characterization of ribosomes from the blue-green alga Anabaena flos-aquae

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Summary

0946 09

  1. 1.

    Ribosomes of the blue-green alga, Anabaena flos-aquae, were isolated using a rapid lysis method and by grinding with alumina.

  2. 2.

    Analysis of ribosomes obtained by rapid lysis on sucrose gradients and electrophoretic gels indicated the presence of 30S, 50S, 70S, and 100S sibosomes as well as polysomes.

  3. 3.

    Dissociation of algal 70S and 100S ribosomes into their 30S and 50S subunits was demonstrated using a 6000xg preparation on a two-dimensional electrophoretic gel as well as a 105000xg fraction on a sucrose gradient.

  4. 4.

    Differences between blue-green algal and bacterial ribosomes were noted when 105000xg fractions of both were washed in ribosomal washing buffer. A major 100S or dimer peak was observed for the bacterial preparation before and after washing. The major peak in the algal preparation shifted from 100S to 70S after washing.

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Abbreviations

O.D.266 :

optical density observed at 260 mμ

S6:

the supernatant fraction obtained by centrifuging cell extracts at 6000xg for five minutes

P105:

the pellet obtained by centrifuging a ribosomal extract at 105000xg for 90 min.

1′↑:

indication of direction of first dimension of gel

2′ ↑:

indication of direction of second dimension of gel

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Authors and Affiliations

  1. University of Delaware, 19711, Newark, Delaware, USA

    John R. Carlton & Diane S. Herson

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  1. John R. Carlton
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  2. Diane S. Herson
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Carlton, J.R., Herson, D.S. The electrophoretic characterization of ribosomes from the blue-green alga Anabaena flos-aquae . Archiv. Mikrobiol. 86, 39–48 (1972). https://doi.org/10.1007/BF00412398

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  • DOI: https://doi.org/10.1007/BF00412398

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