Summary
The fine structure of Micrococcus cryophilus was studied by means of the freeze-etching technique, and compared with previous studies on ultra-thin The freeze-etched cell showed structures similar to those observed in thin sections, the cell wall has a wavy surface structure and consisted of two layers. The cytoplasmic membrane fractured into two parts; one adhering to the cell wall, the other adhering to the cytoplasm; both were covered with spherical particles, 8–12 nm in diameter. In the cytoplasm, nuclear material, mesosomes, and aggregated spherical particles were observed. The results confirm that the cell structure of M. cryophilus is similar to that of Neisseria spp. cells. It should be therefore classified among non-pigmented, asaccharolytic neisserias.
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Bauman, P., Doudoroff, M., Stanier, R. Y.: Study of the Moraxella group. I. Genus Moraxella and the Neisseria catarrhalis group. J. Bact. 95, 58–73 (1968).
Bayer, M. E., Remsen, C. C.: Structure of Escherichia coli after freeze-etching. J. Bact. 101, 304–313 (1970).
Bladen, H. A., Mergenhagen, S. E.: Ultrastructure of Veillonella and morphological correlation of an outer membrane with particles associated with endotoxic activity. J. Bact. 88, 1482–1492 (1964).
Boháček., J., Kocur, M., Martinec, T.: DNA base composition and taxonomy of some micrococci. J. gen. Microbiol. 46, 369–376 (1967).
Bøvre, K., Fiandt, M., Szybalski, W.: DNA base composition of Neisseria, Moraxella and Acinetobacter as determined by buoyant density in CsCl gradients. Cand. J. Microbiol. 15, 335–338 (1969).
Cesarini, J. P., Vanderkerkove, M., Faucon, R., Nicoli, J.: Ultrastructure de la paroi de Neisseria meningitidis. Ann. Inst. Pasteur 113, 833–841 (1967).
Fitz-James, P.: Thin section of dividing Neisseria genorrhoeae J. Bact. 87, 1477–1482 (1964).
Geymayer, W.: Die gleichzeitige elektronenmikroskopische Erfassung von Oberfläche und Querschnitt gefriergeschockter kolloidaler Systeme. VI. Int. Congr. Electron. Microscopy, Kyoto, vol. I, p. 577 (1966).
Girard, A. E., Cosenza, B. J.: A comparison of the fine structure of Micrococcus diversus, Neisseria catarrhalis and Neisseria sicca. Antonie v. Leeuwenhoek 36, 233–244 (1970).
Holt, S. C., Leadbetter, E. R.: Comparative ultrastructure of selected aerobic sporeforming bacteria: A freeze-etching study. Bact. Rev. 33, 346–378 (1969).
Kandler, O., Schleifer, K. H., Niebler, E., Nakel, M., Zahradnik, H., Ried, M.: Murein types in micrococci and similar microorganisms. Publ. Fac. Sci. Univ. J. E. Purkyně, Brno K 47, 143–156 (1970).
Mazanec, K., Kocur, M., Martinec, T.: Electron microscopy of ultrathin sections of Micrococcus cryophilus. Canad. J. Microbiol. 12, 465–469 (1966).
McLean, R. A., Sulzbacher, W. L., Mudd, S.: Micrococcus cryophilus n. sp. a large coccus especially suitable for cytological study. J. Bact. 62, 723–728 (1951).
Meyer, H. W., Winkelman, H.: Die Gefrierätzung und die Struktur, biologischer Membranen. Protoplasma (Wien) 68, 253–270 (1969).
Nanninga, N.: Ultrastructure of the cell envelope of Escherichia coli B after freeze-etching. J. Bact. 101, 297–303 (1970).
Sleytr, U.: Die Gefrierätzung korrespondierender Bruchhälften: ein neurer Weg zur Aufklärung von Membranstrukturen. Protoplasma (Wien) 70, 101–117 (1970a).
—: Fracture faces in intact cells and protoplasts of Bacillus stearothermophilus. A study by conventional freeze-etching and freeze-etching of corresponding fracture moieties. Protoplasma (Wien) 71, 295–312 (1970b).
— Adam H., Klaushofer, H.: Die Feinstruktur der Zellwand und Cytoplasmamembran von Clostridium nigrificans, dargestellt, mit Hilfe der Gefrierätz-und Ultradünnschnittechnik. Arch. Mikrobiol. 66, 40–58 (1969).
— Krebs, B.: Protoplastenbildung und Interpretation des Gefrierätzbildes der Membranstrukturen bei Clostridium nigrificans. Arch. Mikrobiol. 77, 377–389 (1971).
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Sleytr, U., Kocur, M. Structure of Micrococcus cryophilus after freeze-etching. Archiv. Mikrobiol. 78, 353–359 (1971). https://doi.org/10.1007/BF00412275
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DOI: https://doi.org/10.1007/BF00412275