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Rhythmic mycelial growth in Podospora anserina

III. Effect of metabolic inhibitors

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Summary

0725 09

  1. 1.

    Inhibition of the tricarboxylic acid cycle by sodium fluoroacetate (NFA) suppresses the rhythmic growth in the mutant zonata (z) of Podospora anserina and enhances its linear growth rate. However, in the wild strain, the only effect of this inhibitor is a reduction of the linear growth rate.

  2. 2.

    The z-like growth pattern induced in the wild strain by D-galactose also is altered to uniform growth by NFA. The hyphae of the reversed colonies exhibit wild-type like shape and do not branch as intensively as the hyphae of the mutant z under normal growth conditions or of the wild strain growing on D-galactose.

  3. 3.

    The rhythmic growth of z is also prevented, if the respiratory chain is inhibited by sodium azide. In contrast, 2,4-dinitrophenol, which uncouples oxidative phosphorylation and enhances oxidative degradation of substrates, induces rhythmic growth in the wild strain.

  4. 4.

    Chloramphenicol and actidion (cycloheximide) do not prevent rhythmic mycelial growth, but induce this growth pattern in the wild strain.

  5. 5.

    The results indicate that rhythmic growth in Podospora anserina results mainly from an enhancement of oxidative sugar degradation.

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Abbreviations

NFA:

Sodium fluoroacetate

2,4-DNP:

2,4-dinitrophenol

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Authors and Affiliations

  1. Institut für Allgemeine Botanik, Ruhr-Universität Bochum, Germany

    Gernot Lysek

  2. Botanisches Institut, Technische Universität München, BRD-8050, Freising-Weihenstephan, Germany

    Gernot Lysek

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  1. Gernot Lysek
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Lysek, G. Rhythmic mycelial growth in Podospora anserina . Archiv. Mikrobiol. 78, 330–340 (1971). https://doi.org/10.1007/BF00412273

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  • DOI: https://doi.org/10.1007/BF00412273

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