Summary
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1.
An axenic culture of a species of Nostoc from the coralloid roots of the cycad Macrozamia lucida, grows heterotrophically in complete darkness at measurable and reproducible rates.
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2.
Heterotrophic growth was supported by glucose, fructose and sucrose over the pH range 6 to 9. The growth rate with glucose was increased if casamino acids were added.
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Heterotrophic growth on glucose proceeded under both aerobic and anaerobic conditions.
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Cultures grown in the dark were pigmented. There was no significant difference in the ultrastructure of photoautotrophically-grown and dark heterotrophically grown cells.
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Dedicated to Doctor D. S. Hoare, deceased.
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Hoare, D.S., Ingram, L.O., Thurston, E.L. et al. Dark heterotrophic growth of an endophytic blue-green alga. Archiv. Mikrobiol. 78, 310–321 (1971). https://doi.org/10.1007/BF00412271
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DOI: https://doi.org/10.1007/BF00412271