Summary
In an investigation of the role of glucanases in modifying yeast cell walls at the location of new buds, vesicles derived from the endoplasmic reticulum, which are secreted locally into the cell wall of growing buds, and may be involved in the secretion of glucanases, have been isolated.
In yeast, exo-β-1,3-glucanase is present both extra- and intracellularly. Exponentially growing cells contain at least 11% of the enzyme activity intracellularly (within the plasmalemma). Most of this intracellular glucanase is sedimentable. Of the three classes of subcellular particles that contain glucanase, one is almost completely absent from stationary phase cells and largely absent from cells of the late budding phase of the cell cycle. These particles were isolated from budding cells by combined differential and density gradient centrifugation. They contain exo- and endo-β-1,3-glucanases, mannan and protein. The isolate consists mainly of membrane-bounded vesicles with diameters corresponding to those of the secretory vesicles observed in situ. It is concluded that these particles are identical with the vesicles derived from the endoplasmic reticulum.
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Cortat, M., Matile, P. & Wiemken, A. Isolation of glucanase-containing vesicles from budding yeast. Archiv. Mikrobiol. 82, 189–205 (1972). https://doi.org/10.1007/BF00412191
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DOI: https://doi.org/10.1007/BF00412191