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Das Verhalten von Nitratreductase, Nitritreductase, Hydrogenase und anderen Enzymen von Ankistrodesmus braunii bei Stickstoffmangel

Nitrate reductase, nitrite reductase, hydrogenase, and other enzymes in nitrogen-deficient Ankistrodesmus braunii

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Summary

An oxidation of organic nitrogen compounds leading to an intracellular formation of nitrite and nitrate (heterotrophic nitrification) was found in nitrogen-deficient Ankistrodesmus braunii. This explains the rather high levels of nitrate and nitrite reductases observed in algae after the supply of nitrogen has been exhausted.

Hydrogenase is active also in nitrogen-deficient algae which, however, can no longer use nitrite as an acceptor for hydrogen. The activation of hydrogenase is energy-dependent and can be inhibited by means of antibiotics (actinomycin C, puromycin, and gentamycin). Protein synthesis seems to take place during incubation under hydrogen.

For comparison, several other enzymes [glucose-6-phosphate dehydrogenase, NAD(P) reductase, glyoxylate reductase, catalase, malate dehydrogenase, glutamate dehydrogenase, and isocitratase) were studied in nitrogen-deficient cells.

Zusammenfassung

Bei der N-Verarmung von Ankistrodesmus braunii wurde eine intracelluläre Bildung von Nitrit und Nitrat durch heterotrophe Nitrifikation festgestellt, die charakteristisch für den beginnenden N-Mangel ist. Damit wird die hohe Nitrat- und Nitritreductaseaktivität bei N-Mangelalgen verständlich.

Die Hydrogenase ist auch bei N-verarmten Algen aktiv, doch kann Nitrit nicht mehr als H-Acceptor verwendet werden. Die Aktivierung des Enzyms ist energieabhängig und durch Antibiotica (Actinomycin C, Puromycin, Gentamycin) hemmbar. Offenbar findet während der Inkubation mit Wasserstoff eine Proteinsynthese statt.

Zum Vergleich wurde das Verhalten einiger anderer Enzyme [Glucose-6-phosphat-dehydrogenase, NAD(P)-Reductase, Glyoxylat-reductase, Katalase, Malat-dehydrogenase, Glutamat-dehydrogenase, Isocitratase] bei N-Mangel untersucht.

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Abbreviations

G-6-P-DH:

Glucose-6-phosphat-dehydrogenase

M-DH:

Malat-dehydrogenase

Glu-DH:

Glutamat-dehydrogenase

NAD(P)-R:

Nicotinamid-adenin-dinucleotid(phosphat)-reductase

CCCP:

Carbonylcyanid-mchlorophenylhydrazon

DNP:

2,4-Dinitrophenol

MB:

Methylenblau

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  1. Botanisches Institut der Universität, Erlangen

    Heinz Oesterheld

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Oesterheld, H. Das Verhalten von Nitratreductase, Nitritreductase, Hydrogenase und anderen Enzymen von Ankistrodesmus braunii bei Stickstoffmangel. Archiv. Mikrobiol. 79, 25–43 (1971). https://doi.org/10.1007/BF00412038

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  • DOI: https://doi.org/10.1007/BF00412038

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