Abstract
PCR primers derived from porcine short and long interspersed repeat sequences were used to amplify DNA samples isolated from individual members of threegeneration pig reference pedigrees. Subsequent high-resolution gel electrophoresis of both SINE and LINE-PCR products allowed direct visualisation of polymorphisms that segregated in a Mendelian manner. Additional polymorphisms were detected by Southern blotting of the gels described above followed by hybridization with simple sequence DNA. Genotyping by interspersed repeat-PCR exploits the natural architecture of the pig genome and allows the typing of polymorphisms by utilizing pre-existing sequence information.
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Miller, J.R. Use of porcine interspersed repeat sequences in PCR-mediated genotyping. Mammalian Genome 5, 629–632 (1994). https://doi.org/10.1007/BF00411458
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DOI: https://doi.org/10.1007/BF00411458