Abstract
During the sclerotization of microplasmodia of Physarum polycephalum in non-nutrient salt medium or in salt medium supplemented by glucose, RNA or nucleotides a 6-fold increase in the specific activity of an alkaline endonuclease was found within 6 h after the induction. The increase was based on de novo synthesis of the enzyme and it was strongly correlated to the sharp drop in the level of cellular RNA in the first hours of the process of scerotization. The induction in exhausted growth medium or in salt medium supplemented by protein or mannitol showed a gradual 2-3-fold increase of the endonuclease in 30 h, parallel to the gradual decrease of the RNA. No changes in the specific activity of the endonuclease were found during logarithmic growth or under conditions of starvation without the induction to sclerotization.
The alkaline, polyA-specific endonuclease could possibly regulate the turnover of RNA.
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References
Adams, D. S., Jeffery, W. R.: Poly(A) degradation by two distinct processes in the cytoplasmic RNA of Physarum polycephalum. Biochemistry 17, 4519–4524 (1978)
Brand, G., Hüttermann, A.: Activity and isoenzymes of RNases during starvation induced spherulation of Physarum polycephalum. Abstr. Physarum Meeting, Göttingen, pp. 36–37 (1972)
Chet, I., Rusch, H. P.: Induction of spherule formation in Physarum polycephalum. J. Bacteriol. 100, 673–678 (1969)
Chet, I., Rusch, H. P.: RNA differences between spherulating and growing microplasmodia of Physarum polycephalum as revealed by sedimentation pattern and DNA-RNA hybridization. Biochim. Biophys. Acta 209, 559–568 (1970a)
Chet, I., Rusch, H. P.: RNA and protein synthesis during germination of spherules of Physarum polycephalum. Biochim. Biophys. Acta 224, 620–622 (1970b)
Chet, I., Retig, N., Henis, Y.: Changes in RNases during differentiation (spherulation) of Physarum polycephalum. Biochim. Biophys. Acta 294, 343–347 (1973)
Chet, I.: Changes in RNA during differentiation of Physarum polycephalum. Ber. Deutsch. Bot. Ges 86, 77–92 (1973)
Daniel, J. W., Baldwin, H. H.: Methods of culture for plasmodial myxomycetes. In: Methods in Cell Physiol., Vol. 1 (D. M. Prescott, ed.), pp. 9–42. New York: Academic Press 1964
Goodman, E. M., Beck, T.: Metabolism during differentiation in the slime mold Physarum polycephalum. Can. J. Microbiol. 20, 107–111 (1974)
Hüttermann, A., Porter, M. T., Rusch, H. P.: Activity of some enzymes in Physarum polycephalum. II. Arch. Mikrobiol. 74, 283–291 (1970)
Hüttermann, A., Elsevier, S. M., Eschrich, W.: Evidence for the de novo synthesis of glutamate dehydrogenase during the spherulation of Physarum polycephalum. Arch. Mikrobiol. 77, 74–85 (1971)
Hüttermann, A., Chet, I.: Activity of some enzymes in Physarum polycephalum. III. Arch. Mikrobiol. 78, 189–192 (1971)
Hüttermann, A.: Isoenzyme pattern and de novo synthesis of phosphodiesterase during differentiation (spherulation) in Physarum polycephalum. Arch. Mikrobiol. 83, 155–164 (1972)
Hüttermann, A., Gebauer, M.: Differential protein synthesis during differentiation (spherulation) of Physarum polycephalum: lack of synthesis of glucose-6-phosphate dehydrogenase. Arch. Mikrobiol. 85, 91–94 (1972)
Hüttermann, A.: Biochemical events during spherule formation of Physarum polycephalum. Ber. Deutsch. Bot. Ges. 86, 55–76 (1973)
Hüttermann, A., Gebauer, M., Brand, G., Wessel, I.: Studies on the regulation of carbohydrate synthesis during growth and differentiation of Physarum polycephalum. I. Arch. Microbiol. 98, 215–223 (1974)
Kikuchi, M.: Studies on sclerotia of a myxomycete Physarum polycephalum I. Sc. Rep. T. K. D. Sect. B 14, 215–226 (1971)
Knowles, D. J. C., Carlile, M. J.: Growth and migration of plasmodia of the myxomycete Physarum polycephalum: the effect of carbohydrates, including agar. J. gen. Microbiol. 108, 9–15 (1978)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)
McAlister, L. E., Allison, V. F., Jeter, J. R., Nations, C.: Highdensity induction of a quiescent cell state in Physarum polycephalum. J. Cell Sci. 25, 179–190 (1977)
McCormick, J. J., Blomquist, J. C., Rusch, H. P.: Isolation and characterization of an extracellular polysaccharide from Physarum polycephalum. J. Bacteriol 104, 1110–1118 (1970a)
McCormick, J. J., Blomquist, J. C., Rusch, H. P.: Isolation and characterization of a galactosamine wall from spores and spherules of Physarum polycephalum. J. Bacteriol. 104, 1119–1125 (1970b)
Nations, C., LeStourgeon, W. M., Magun, B. E., Rusch, H. P.: The rapid intranuclear accumulation of preexisting proteins in response to high plasmodial density in Physarum polycephalum. Exp. Cell Res. 88, 207–215 (1974)
Pieck, A. C. M., Kuyper, Ch. M. A., Rijken, A. A. M.: Comparison of the pool of nucleotides from liver cells under different physiological conditions. Proc. Koninkl. Acad. Wetensch. (Amsterdam) 74, 311–317 (1971)
Polman, B. J. J.: DNA degrading enzymes of the slime mold Physarum polycephalum Schwein. Ph. D. Thesis, Nijmegen (1974)
Polman, B. J. J., Janssen, H. M. J., Kuyper, Ch. M. A.: Activities of DNA degrading enzymes in the slime mold Physarum polycephalum. Arch. Microbiol. 96, 119–124 (1974)
Sachsenmaier, W., Rusch, H. P.: The effect of 5-fluoro-2′-deoxyuridine on synchronous mitosis in Physarum polycephalum. Exp. Cell Res. 36, 124–133 (1964)
Sauer, H. W., Babcock, K. L., Rusch, H. P.: Changes in nucleic acid and protein synthesis during starvation and spherule formation in Physarum polycephalum. Arch. Entwicklungsmech. Organ. 165, 110–124 (1970)
Wendelberger-Schieweg, G., Hüttermann, A.: Amino acid pool and protein turnover during differentiation (spherulation) of Physarum polycephalum. Arch. Microbiol. 117, 27–34 (1978)
Werry, P. A. T. J.: DNA synthesis in Physarum polycephalum. Ph. D. Thesis, Nijmegen (1973)
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Waterborg, J.H., van Brakel, H.F.W. & Kuyper, C.M.A. Differential synthesis of an alkaline endonuclease in Physarum polycephalum . Arch. Microbiol. 122, 195–200 (1979). https://doi.org/10.1007/BF00411360
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DOI: https://doi.org/10.1007/BF00411360