Summary
After pulse-labelling with 32P-orthophosphate and fractionation of the nucleic acids from synchronously cultured cells of the green alga Chlorella pyrenoidosa on methylated serum albumin and kieselgur (MAK) the DNA contained a species of 32P-RNA. About 3% of the total 32P radioactivity incorporated in the cells' RNA were confined to this DNA-associated component. Its base ratio differed significantly from that of soluble and ribosomal RNA but varied only slightly during the life-cycle of the cells. About 4% of the DNA-associated 32P-RNA resisted ribonuclease digestion suggesting a stable binding of RNA to DNA in the form of a complex. Gel filtration and sucrose gradient centrifugation of the nucleic acids isolated in the DNA region during previous MAK column chromatography resulted in a separation of most of the 32P-RNA from the DNA. The remaining ribonuclease-resistent but alkali labile 32P radioactivity bound to the latter was in the order of 4%. No evidence has been obtained so far that it represents rapidly synthesized RNA associated with DNA in stable and functional complex.
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Reiderbeck, R., Richter, G. Characterization of rapidly labelled RNA associated with DNA in Chlorella . Archiv. Mikrobiol. 67, 256–272 (1969). https://doi.org/10.1007/BF00411261
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DOI: https://doi.org/10.1007/BF00411261