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Synchronous cell divisions in Anacystis nidulans Richter

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Summary

The blue-green alga, Anacystis nidulans was successfully synchronized in continuous light (15,000 lux) by a 14 hr cycle, consisting of 8 hrs at 26°C, and 6 hrs at 32°C, coupled with periodic dilution of the algal suspension to a constant cell number at the end of each cycle. The alga continues to grow at the lower temperature, and a “division burst” begins 2 hrs after transfer to the higher temperature.

Zusammenfassung

Die Cyanophycee Anacystis nidulans wurde in Dauerlicht von 15 000 Lux vollständig synchronisiert. Die Cyclen bestehen aus 8 Std bei 26° C und 6 Std bei 32° C, an ihrem Ende wurde jeweils mit frischer Nährlösung auf die Ausgangszellzahl von 3·107 Zellen/ml verdünnt. Bei beiden Temperaturen kann sich die Alge vermehren, unter den synchronisierenden Bedingungen des Temperaturwechsels beginnt der Teilungsschub (Verdopplung der Zellzahl) bei 32° C nach 2 Std.

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Author notes

  1. G. S. Venkataraman

    Present address: Division of Microbiology, Indian Agricultural Research Institute, Delhi-12, India

Authors and Affiliations

  1. Institute of Plant Physiology, University of Göttingen, Gottingen, Germany

    Harald Lorenzen & G. S. Venkataraman

Authors
  1. Harald Lorenzen
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  2. G. S. Venkataraman
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Additional information

Pringsheim (1968) renamed this alga as Lauterbornia nidulans.

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Lorenzen, H., Venkataraman, G.S. Synchronous cell divisions in Anacystis nidulans Richter. Archiv. Mikrobiol. 67, 251–255 (1969). https://doi.org/10.1007/BF00411260

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  • DOI: https://doi.org/10.1007/BF00411260

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