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Cloning of DNA sequences from Methanococcus vannielii capable of autonomous replication in yeast

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Abstract

Total DNA of the archaebacterium Methanococcus vannielii was digested with BamHI or BamHI/HindIII, cloned with plasmid Yip5 and analyzed for sequences capable of autonomous replication (ARSs) in the eukaryote Saccharomyces cerevisiae. Two recombinant plasmids were isolated which contained 3.3 kb and 8 kb fragments of methanogen derived DNA with ARS activity. They exhibited low transformation efficiencies for yeast and promoted slow growth of yeast transformants.

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Abbreviations

Ap:

ampicillin

ARS:

autonomously replicating sequence

EtBr:

ethidium bromide

kb:

kilobase(s)

Mc.:

Methanococcus

R:

resistance

RE:

replication enhancer

RS:

replication sequence

Tc:

tetracycline

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Authors and Affiliations

  1. Mikrobiologisches Institut der Eidgenössischen Technischen Hochschule Zürich, CH-8092, Zürich, Switzerland

    Leo Meile & Thomas Leisinger

  2. Department of Microbiology, The Ohio State University, 43210, Columbus, OH, USA

    John N. Reeve

Authors
  1. Leo Meile
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  2. Thomas Leisinger
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  3. John N. Reeve
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Meile, L., Leisinger, T. & Reeve, J.N. Cloning of DNA sequences from Methanococcus vannielii capable of autonomous replication in yeast. Arch. Microbiol. 143, 253–255 (1985). https://doi.org/10.1007/BF00411245

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  • DOI: https://doi.org/10.1007/BF00411245

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