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Cellulase production with Penicillium iriense (n. sp.)

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Summary

A new cellulase producing species of penicillium, named Penicillium iriense, has been isolated. Cultures of this fungus in liquid media containing cellulose as carbon source. excrete into the medium an enzyme complex able to degrade both soluble and insoluble forms of cellulose. This complex has been separated into five protein fractions. Three of them are endowed with CM-cellulase activity, one contains a cellobiase and one contains a C1-like factor. These fractions show a moderate synergism in the attack of cotton fibres.

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References

  • Gascoigne, J. A., Gascoigne, M. M.: Biological degradation of cellulose. London: Buttersworth 1960

    Google Scholar 

  • Halliwell, G.: The action of cellulolytic enzyme from Myrothecium verrucaria. Biochem. J. 79, 185–192 (1961)

    Google Scholar 

  • Halliwell, G., Riaz, M.: The formation of short-fibres from native cellulose by components of Trichoderma koningii cellulase. Biochem. J. 116, 35–42 (1970)

    Google Scholar 

  • Halliwell, G., Riaz, M.: Interactions between components of the cellulase complex of Trichoderma koningii on native substrates. Arch. Mikrobiol. 78, 295–309 (1971)

    Google Scholar 

  • Hash, J. H., King, K. W.: On the nature of the glucosidases of Myrothecium verrucaria. J. biol. Chem. 232, 381–393 (1958)

    Google Scholar 

  • King, K. W., Vessal, M. I.: Enzymes of the cellulase complex. In: Cellulases and their applications, Advances in Chemistry, series 95, F. Gould, Ed., pp. 7–25. Washington: A.C.S. 1969

    Google Scholar 

  • Konno, N., Amermura, A., Kokumai, K., Terui, G.: Components of the cellulase system from Penicillium variabile. Hakko Kogaku Zasshi 41, 385–390 (1963) (Chem. Abstr. 63 4590h)

    Google Scholar 

  • Lindwall, S., Eriksson, A. F.: Studies on a new cellulase preparation from Penicillium. II. Properties and action upon different substrates. J. Pharm. Pharmacol. XI, 756–762 (1959)

    Google Scholar 

  • Lowry, H., Rosebrough, N. J., Lewis Farr, A., Randall, R. J.: Protein measurement with the Folin Phenol Reagent. J. biol. Chem. 193, 265–275 (1951)

    Google Scholar 

  • Mandels, M., Weber, J.: The production of cellulases. In: Cellulases and their applications. Advances in Chemistry, series 95, F. Gould, Ed., pp. 391–414 Washington: A.C.S. 1969

    Google Scholar 

  • Mazzucchetti, G.: Microfunghi della cellulosa a della carta. Attività e inquadramento sitematico. II genere “Chetomium”. Roma: Pubbl. Ente Naz. per la cellulosa e la carta 1965

    Google Scholar 

  • Nelson, N.: A photometric adaptation of the Somogyi method for the determination of glucose. J. biol. Chem. 153, 375–380 (1944)

    Google Scholar 

  • Ogur, M., Rosen, G.: The nucleic acids of plant tissues. I. The extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid. Arch. Biochem. 25, 262–276 (1950)

    Google Scholar 

  • Peterson, E. A., Sober, H. A.: Variable gradient device for chromatography. Analyt. Chem. 31, 857–862 (1959)

    Google Scholar 

  • Pettersson, G.: Separation of cellulases on Sephadex G-100. Biochim. biophys. Acta (Amst.) 77, 665–667 (1963)

    Google Scholar 

  • Pettersson, G.: Isolation of a cellulase from Penicillium notatum. Arch. Biochem. Biophys. 123, 307–311 (1968)

    Google Scholar 

  • Raper, K. B., Thom, C.: A manual of the Penicillia, pp. 64–68. Baltimore: The Williams & Wilkins Co. 1949

    Google Scholar 

  • Russel, J. A.: The colorimetric estimation of small amounts of ammonia by the phenol hypochlorite reaction. J. biol. Chem. 156, 457–461, (1944)

    Google Scholar 

  • Selby, K.: The purification and properties of the C1 component of the cellulase complex. In: Cellulase and their applications, Adv. in Chemistry, series 95, F. Gould, Ed., pp. 34–52. Washington: A.C.S. 1969

    Google Scholar 

  • Selby, K., Maitland, C. C.: The cellulase of Trichoderma viride. Separation of its components involved in the solubilization of cotton. Biochem. J. 104, 716–724 (1967)

    Google Scholar 

  • Somogyi, M.: Notes on sugar determination. J. biol. Chem. 195, 19–23 (1952)

    Google Scholar 

  • Tribe, H. T.: Decomposition of buried cellulose film, with special reference to the ecology of certain soil fungi. In: The ecology of soil fungi, D. Parkinson, J. S. Waid, Eds., pp. 246–256. Liverpool: Univ. Press 1960

    Google Scholar 

  • Wood, T. M.: Cellulolytic enzyme system of Trichoderma koningii (separation of components attacking native cotton). Biochem. J. 109, 217–227 (1968)

    Google Scholar 

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Boretti, G., Garofano, L., Montecucchi, P. et al. Cellulase production with Penicillium iriense (n. sp.). Archiv. Mikrobiol. 92, 189–200 (1973). https://doi.org/10.1007/BF00411199

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  • DOI: https://doi.org/10.1007/BF00411199

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