Skip to main content
Log in

Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase

  • Short Communications
  • Published:
Current Genetics Aims and scope Submit manuscript


Aspergillus niger transformation frequencies of up to 1,176 transformants per μg DNA were achieved using the plasmid vector pSTA10 containing the A. niger nitrate reductase structural gene. Analysis of genomic endonuclease cleaved DNA from nitrate utilising transformants by DNA hybridisation, showed that most integration events are as a result of homologous recombination. The niaD transformation system was used successfully for the introduction of the unselected Escherichia coli fusion genes lacZ, encoding β-galactosidase, and uidA, for β-glucuronidase, as well as the Neurospora crassa tub-2 gene, for β-tubulin. pSTA10 was also capable of transforming niaD mutants of other filamentous fungi such as A. nidulans, A. oryzae and Penicillium chrysogenum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.


  • Buxton FP, Gwynne DE, Davis RW (1985) Gene 37:207–214

    Google Scholar 

  • Goosen T, Bloomheuvel G, Gysler C, De Bie DA, Van den Broek HWJ, Swart K (1987) Curr Genet 11:499–505

    Google Scholar 

  • Kelly JM, Hynes MJ (1985) EMBO J 4:475–479

    Google Scholar 

  • Orbach MJ, Porro EB, Yanofsky C (1986) Mol Cell Biol 6:2452–2461

    Google Scholar 

  • Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CAMJJ (1987) Gene 56:117–124

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning — a laboratory manual, Cold Spring Harbor Laboratory, New York, USA

    Google Scholar 

  • Roberts IN, Oliver RP, Punt PJ, van den Hondel CAMJJ (1989) Curr Genet 15:177–180

    Google Scholar 

  • Tilburn T, Scazzocchio C, Taylor GG, Zabicky-Zissman JH, Lockington RA, Davies RW (1983) Gene 26:205–221

    Google Scholar 

  • Tomsett AB, Cove DJ (1979) Gen Res Camb 34:19–32

    Google Scholar 

  • Unkles SE (1989) In: Wray JL, Kinghorn JR (eds) Molecular and genetic aspects of nitrate assimilation. Chapter 22, Oxford University Press, Oxford, UK

    Google Scholar 

  • Unkles SE, Campbell EI, Carrez D, Grieve C, Contreras R, van den Hondel CAMJJ, Kinghorn JR (1989a) Gene 78:157–166

    Google Scholar 

  • Unkles SE, Campbell EI, de Ruiter-Jacobs YMJT, Broekhuijsen M, Macro JA, Carrez D, Contreras R, van den Hondel CAMJJ, Kinghorn JR (1989b) Mol Gen Genet 218:999–104

    Google Scholar 

  • Van Gorcom REM, Pouwels PH, Goosen T, Viser J, van den Broek HWK, Hamer JE, Timberlake WE, van den Hondel CAMJJ (1985) Gene 40:99–106

    Google Scholar 

  • Van Hartingsveldt W, Matter IE, Van Zeijl CMJ, Pouwels PH, van den Hondel CAMJJ (1987) Mol Gen Genet 206:71–75

    Google Scholar 

  • Ward M, Wilson LJ, Carmona CL, Turner G (1988) Curr Genet 14:37–42

    Google Scholar 

  • Whitehead MP, Unkles SE, Ramsden M, Campbell E, Gurr SJ, Spence D, Kinghorn JR (1989) Mol Gen Genet 216:408–411

    Google Scholar 

  • Yelton MM, Hamer JE, Timberlake WE (1984) Proc Natl Acad Sci USA 81:1470–1474

    Google Scholar 

Download references

Author information

Authors and Affiliations


Rights and permissions

Reprints and permissions

About this article

Cite this article

Campbell, E.I., Unkles, S.E., Macro, J.A. et al. Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase. Curr Genet 16, 53–56 (1989).

Download citation

  • Received:

  • Issue Date:

  • DOI:

Key words