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Free amino acid pools of Trichoderma aureoviride during conditions of glucose-limited growth and glucose starvation

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Abstract

Glucose-limited and glucose-starved cultures of Trichoderma aureoviride were analyzed for the size and composition of the mycelial free amino acid pool. In glucoselimited mycelia the pool size increased as a function of the specific growth rate above a value of ca. 0.08 h-1 and this was due principally to increasing concentrations of alanine and glutamic acid. During glucose starvation, the net pool size decreased only by ca 20% although a transient elevation of free amino acids was observed, the latter being attributed to the turnover of mycelial proteins. The amino acid pool compositions were categorized according to their ionic nature and, although no particular group varied significantly in its percentage contribution to the total pool size of growing mycelia, the observed variations during starvation were mostly attributable to the basic and acidic amino acids. Comparisons are made of the results with those obtained for other species of filamentous fungi and some possible explanations for the observed variations are discussed.

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References

  • Blackburn S (1968) Amino acid determination; methods and techniques. London, Edward Arnold

    Google Scholar 

  • Brown CM, Stanley SO (1972) Environment mediated changes in the cellular content of the “pool” constituents and their associated changes in cell physiology. J Appl Chem Biotech 22:363–389

    Google Scholar 

  • Carter BLA, Bull AT (1969) Studies of fungal growth and intermediary carbon metabolism under steady and non-steady state conditions. Biotechnol Bioeng 11:785–804

    Google Scholar 

  • Chesters CGC, Bull AT (1963) The enzyme degradation of laminarin. Biochem J 86:28–46

    Google Scholar 

  • Cowie DB, McClure FT (1959) Metabolic pools and the synthesis of macromolecules. Biochim Biophys Acta 31:236–245

    Google Scholar 

  • Dawson PSS (1965) The intracellular amino acid pool of Candida utilis during growth in batch and continuous flow cultures. Biochim Biophys Acta 111:51–66

    Google Scholar 

  • Holden JT (1962) The composition of microbial amino acid pools. In: JT Holden (ed) Amino acid pools. Elsevier, Amsterdam, pp 73–108

    Google Scholar 

  • Jaworski A, Diugonski J, Wilmanska D Sedlaczek L (1976) Changes in the cellular content of the pool constituents of Monosporium olivaceum — a steroid hydroxylating mould. Acta Microbiol Polonica 25:329–335

    Google Scholar 

  • Kubicek CP, Hampel W, Rohr M (1979) Manganese deficiency leads to elevated amino acid pools in citric acid accumulating Aspergillus niger. Arch Microbiol 123:73–79

    Google Scholar 

  • Lehninger AL (1975) Biochemistry. Worth Publishers Inc., New York

    Google Scholar 

  • McGetrick AMT, Bull AT (1979) Phenotypic changes in the chemistry of Aspergillus nidulans: influence of culture conditions on mycelial composition. Arch Microbiol 123:151–156

    Google Scholar 

  • Pitt DE (1979) Metabolic and morphological aspects of growth and survival of Trichoderma aureoviride. Ph D Thesis, University of Wales

  • Rifai MA (1969) A revision of the genus Trichoderma. Mycol Paper No. 116. Commonwealth Mycological Institute, Kew, England

    Google Scholar 

  • Rowley BI, Bull AT (1973) Chemostat for the cultivation of moulds. Laboratory Practice 22:286–289

    Google Scholar 

Download references

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Pitt, D.E., Bull, A.T. Free amino acid pools of Trichoderma aureoviride during conditions of glucose-limited growth and glucose starvation. Arch. Microbiol. 130, 180–184 (1981). https://doi.org/10.1007/BF00411074

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  • DOI: https://doi.org/10.1007/BF00411074

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