Abstract
The conditions under which an oscillatory behaviour is observed during net hydrolysis or synthesis of ATP in chromatophores of Rhodospirillum rubrum FR1 are described. In the case of ATPase the oscillations are observed at low temperature (ca. 11°C) in the dark after an initial transient behaviour. These oscillations are attenuated or disappear by the addition of an uncoupler.
Oscillations are also observed during ATP synthesis. At 3°C the oscillations appear spontaneously if photophosphorylation is measured during a sufficiently long time. At 30°C the mere intercalation of a dark period also at 30°C is sufficient to trigger the oscillations in the following light period.
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Abbreviations
- Bchl:
-
Bacteriochlorophyll
- FCCP:
-
carbonyl cyanide p-trifluoromethoxyphenyl hydrazone
- PMS:
-
phenazine methosulfate
- TMPD, N,N,N′,N′:
-
tetramethyl-1,4-phenylenediamine
References
Bauerlein F (1984) Evidence for the direct mechanism of ATP synthesis of H+-ATPases. In: Schäfer G, et al. (eds) 3rd European Bioenergetics Conference, vol 3B. Congress Edition, Hannover (FRG), pp 739–740
Baccarini-Melandri A, Melandri BA (1971) Partial resolution of the photophosphorylating systems of Rhodopseudomonas capsulata. In: San Pietro A (ed) Methods in enzymology, vol 23. Academic Press, London, pp 556–561
Beard WA, Dilley RA (1984) A method for distinguishing localized from bulk phase proton pool using post-illumination ATP formation after single turnover flashes. In: Schäfer G et al. (eds) 3rd European Bioenergetics Conference, vol 3A. Congress Edition, Hannover (FRG), pp 221–222
Clayton RK (1966) Spectroscopic analysis of bacteriochlorophylls in vitro and in vivo. Photochem Photobiol 5:669–688
Cross RL, Grubmeyer Ch, Penefsky HS (1982) Mechanism of ATP hydrolysis by beef heart mitochondrial ATPase. Rate enhancement resulting from cooperative interactions between multiple catalytic sites. J Biol Chem 257:12101–12106
Decroly O, Goldbeter A (1982) Biorhythmicity, chaos and other patterns of temporal self organization in a multiply regulated biochemical system. Proc Natl Acad Sci USA 79:6917–6921
Dilley RA, Schreiber U (1984) Correlation between membranelocalized protons and flash driven ATP formation in chloroplasts thylakoids. J Bioenerg Biomem 16:173–193
Fernández Velasco J, Romero O, Pucheu NL, García AF (1982) Irreversible inhibition of photophosphorylation by low temperature in Rhodospirillum rubrum chromatophores. Photobiochem Photobiophys 4:39–46
Fernández Velasco J, Romero O, Benzacar NL, Pucheu NL, García AF (1984) Kinetic behaviour of the Mg-ATPase activity in Rhodospirillum rubrum chromatophores. Photobiochem Photobiophys 8:251–258
Frenkel R (1968) Control of reduced diphosphopyridine nucleotide oscillations in beef heart extracts. Effects of modifiers of phosphofructokinase activity. Arch Biochem Biophys 125:151–156
Gerisch G, Wick U (1975) Intracellular oscillations and release of cyclic AMP from Dictyostelium cells. Biochem Biophys Res Commun 65:364–370
Goldbeter A, Caplan SR (1976) Oscillatory enzymes. Ann Rev Biophys Bioenerg 5:449–476
Gould JM, Cather R, Winget GD (1972) Advances on the use of Cerenkov counting for the determination of 32P in photophosphorylation research. Anal Biochem 50:540–548
Gresser MJ, Myers JA, Boyer PD (1982) Catalytic site cooperativity of beef heart mitochondrial F1 adenosine triphosphatase. Correlations of initial velocity, bound intermediate and oxygen exchange measurements with an alternating three site model. J Biol Chem 257:12030–12038
Hess B, Boiteux A (1968) Control of glycolysis. In: Järnefelt J (ed) Regulatory function of biological membranes. Elsevier, Amsterdam, pp 148–162
Kuschmitz D, Hess B (1984) Interaction between the plasma membrane and glycolysis in yeast cells. In: Schäfer G, et al. (eds) 3rd European Bioenergetics Conference, vol 3B. Congress Edition, Hannover (FRG), pp 491–492
Laszlo JA, Baker GM, Dilley RA (1984) Non-equilibration of membrane-associated protons with the internal aqueous space in dark maintained chloroplasts thylakoids. J Bioenerg Biomem 16:37–51
Markus M, Hess B (1984) Transitions between oscillatory modes in a glycolytic model system. Proc Natl Acad Sci USA 81:4394–4398
Markus M, Kuschmitz D, Hess B (1984) Chaotic dynamics in yeast glycolysis under periodic substrate input flux. FEBS Lett 172:235–238
May RM (1976) Simple mathematical models with very complicated dynamics. Nature (Lond) 261:459–467
Pucheu NL, Romero O, Fernández Velasco J, García AF (1982) Kinetic behaviour of the energy transducing system of Rhodospirillum rubrum chromatophores treated under photophosphorylating conditions at low temperature. Photobiochem Photobiophys 4:169–175
Pye EK (1969) Biochemical mechanism underlying the metabolic oscillations in yeast. Can J Bot 47:271–285
Sugino Y, Miyoshi Y (1964) The specific precipitation of orthophosphate and some biochemical applications. J Biol Chem 239:2360–2364
Taussky HH, Shorr E (1954) A microcolorimetric method for the determination of inorganic phosphorus. J Biol Chem 202:675–685
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Dedicated to Prof. Dr. Gerhart Drews as a homage for his permanent example as hard worker and careful scientist and also for his remarkable human quality
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Velasco, J.G.F., Pucheu, N.L., Romero, O. et al. Occurrence of oscillations in ATP synthesis and hydrolysis in chromatophores of Rhodospirillum rubrum . Arch. Microbiol. 143, 192–195 (1985). https://doi.org/10.1007/BF00411046
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DOI: https://doi.org/10.1007/BF00411046