Abstract
R68.45 mediated mobilisation of the chromosome of Methylobacterium sp strain AM1 has been investigated. High frequencies of cotransfer of four genes required for C-1 metabolism with the genes coding for streptomycin, phosphonomycin and cycloserine resistance were demonstrated. A preliminary map of this region has been constructed on the basis of the results of three and four factor crosses showing that not all the C-1 genes are contiguous.
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Abbreviations
- Str:
-
streptomycin
- Pho:
-
phosphonomycin
- Cyc:
-
cycloserine
- Tc:
-
tetracycline
- Km:
-
kanamycin
- Cb:
-
carbenicillin
- Ade:
-
adenine
- Thi:
-
thiamine
- Met:
-
methionine
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Tatra, P.K., Goodwin, P.M. Mapping of some genes involved in C-1 metabolism in the facultative methylotroph Methylobacterium sp strain AM1 (Pseudomonas AM1). Arch. Microbiol. 143, 169–177 (1985). https://doi.org/10.1007/BF00411043
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DOI: https://doi.org/10.1007/BF00411043