Abstract
Filaments without gas vacuoles arose spontaneously in the gas-vacuolate alga Anabaena flos-aquae. The non-vacuolate mutant was enriched by repeated sedimentation and subsequently cloned by microsyringe transfer. No revertants have been observed. In the gas-vacuolate wild-type alga the gas vesicle protein was clearly distinguished by gel electrophoresis as one of the ten most abundant protein species present in whole cell extracts. Electrophoresis indicated that the mutant had lost the ability to synthesize the gas vesicle protein. A second mutant partially defective in production of gas vacuoles and gas vesicle protein has been isolated.
Similar content being viewed by others
Abbreviations
- gv:
-
gas vesicle protein
- pb:
-
phycobilin
- TCA:
-
trichloracetic acid
References
Baum, H., Silman, H. I., Rieske, J. S., Lipton, S. N.: On the composition and structural organization of complex III of the mitochondrial electron transfer chain. J. biol. Chem. 242, 4876 (1967)
Buckland, B., Walsby, A. E.: A study of the strength and stability of gas vesicles isolated from a blue-green alga. Arch. Mikrobiol. 79, 327–337 (1971)
Chapman, D. J.: Biliproteins and bile pigments. In: The biology of blue-green algae (N. G. Carr, B. A. Whitton, eds.), pp. 162–185. Oxford: Blackwell 1973
Das, B., Singh, P. K.: Isolation of gas vacuole-less mutants of the blue-green alga Anabaenopsis raciborskii. Arch. Microbiol. 111, 195–196 (1976)
Dinsdale, M. T., Walsby, A. E.: The interrelations of cell turgor pressure, gas-vacuolation, and buoyancy in a blue-green alga. J. exp. Bot. 23, 561–570 (1972)
Dunker, A. K., Rueckert, R. R.: Observations on molecular weight determinations on polyacrylamide gel. J. biol. Chem. 244, 5074–5080 (1969)
Falkenberg, P., Buckland, B., Walsby, A. E.: Chemical composition of gas vesicles isolated from Anabaena flos-aquae. Arch. Mikrobiol. 85, 309–314 (1972)
Glazer, A. N., Bryant, D. A.: Allophycocyanin B (λmax 671, 618 nm). A new cyanobacterial phycobiliprotein. Arch. Microbiol. 104, 15–22 (1975)
Grant, N. G., Walsby, A. E.: The contribution of photosynthate to turgor pressure rise in the blue-green alga Anabaena flos-aquae. J. exp. Bot. 28, 409–415 (1977)
Jones, D. D., Jost, M.: Isolation and chemical characterization of gas-vacuole membrane from Microcystis aeruginosa. Kuetz. emend. Elenkin. Arch. Mikrobiol. 70, 43–64 (1970)
Jones, D. D., Jost, M.: Characterization of the protein from gasvacuole membranes of the blue-green alga, Microcystis aeruginosa. Planta (Berl.) 100, 277–287 (1971)
Larsen, H., Omang, S., Steensland, H.: On the gas vacuoles of the halobacteria. Arch. Mikrobiol. 59, 197–203 (1967)
Shear, H., Walsby, A. E.: An investigation into the possible lightshielding role of gas vacuoles in a planktonic blue-green alga. Brit. phycol. J. 10, 241–251 (1975)
Stoeckenius, W., Kunau, W. H.: Further characterization of particulate fractions from lysed cell envelope of Halobacterium halobium and isolation of gas vacuole membranes. J. Cell Biol. 38, 336–357 (1968)
Walsby, A. E.: The permeability of blue-green algal gas-vacuole membranes to gas. Proc. roy. Soc. B 173, 235–255 (1969)
Walsby, A. E.: Structure and function of gas vacuoles. Bact. Rev. 36, 1–32 (1972)
Walsby, A. E.: A portable apparatus for measuring relative gas vacuolation, the strength of gas vacuoles, and turgor pressure in planktonic blue-green algae and bacteria. Limnol. Oceanogr. 18, 653–658 (1973)
Walsby, A. E.: The buoyancy-providing role of gas vacuoles in an aerobic bacterium. Arch. Microbiol. 109, 135–142 (1976)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Walsby, A.E. Absence of gas vesicle protein in a mutant of Anabaena flos-aquae . Arch. Microbiol. 114, 167–170 (1977). https://doi.org/10.1007/BF00410779
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00410779