Abstract
From cells of Saccharomyces cerevisiae a factor has been extracted that destroys the agglutinability of a mating-type cells specifically. It was found in the cell extracts of diploid and tetraploid strains as well as haploid strains of a and α mating types. It is heat-labile and the molecular weight is about 50000. It is adsorbed by neither a cells nor α cells. Its biological activity is dependent on the incubation temperature and the pH, and is completely inhibited by phenylmethylsulfonyl fluoride, a potent inhibitor of the serine proteases. All the results described in this paper indicate that this factor is a proteolytic enzyme.
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Shimoda, C., Matsushima, Y. & Yanagishima, N. Mating reaction in Saccharomyces cerevisiae. X Agglutinability-inactivating factor: A factor which destroys sexual agglutinability of a mating-type cells. Antonie van Leeuwenhoek 42, 511–521 (1976). https://doi.org/10.1007/BF00410182
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DOI: https://doi.org/10.1007/BF00410182