Summary
Nitrogenase of Anabaena flos-aquae was inactivated by oxygen and recovery of activity was measured in batch and iron, phosphate and urea-limited continuous cultures. In batch culture, canavanine, chloramphenicol, methylamine, proflavine, puromycin and urea inhibited the recovery process. The rate of recovery of nitrogenase activity in continuous cultures was dependent on light intensity, concentration of urea, ammonium salts and nitrate, and independent of growth rate. Oxygen and urea caused an inactivation of nitrogenase in continuous cultures.
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Bone, D. H.: Relationship between phosphates and alkaline phophatases of Anabaena flos-aquae in continuos culture. Arch. Mikrobiol. 80, 147–153 (1971a).
—: Nitrogenase activity and nitrogen assimilation in Anabaena flos-aquae growing in continuous culture. Arch. Mikrobiol. 80, 234–241 (1971b).
Burris, R. H.: Progress in the biochemistry of nitrogen fixation. Proc. roy. Soc. B 172, 339–354 (1969).
Dalton, H., Postgate, J. R.: Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous culture. J. gen. Microbiol. 54, 463–475 (1969).
Drozd, J., Postgate, J. R.: Effects of oxygen on acetylene reduction, cytochrome content and respiratory activity of Azotobacter chroococcum. J. gen. Microbiol. 63, 63–73 (1970).
Fay, P., Cox, R. M.: Oxygen inhibition of nitrogen fixation in cell-free preparations of blue-green algae. Biochim. biophys. Acta (Amst.) 143, 562–569 (1967).
Good, N. E.: Activation of hill reaction by amines. Biochim. biophys. Acta (Amst.) 40, 502–517 (1960).
Haystead, A., Robinson, R., Stewart, W. D. P.: Nitrogenase activity in extracts of heterocystous and non-heterocystous blue-green algae. Arch. Mikrobiol. 74, 235–243 (1970).
Holzer, H.: Regulation of enzymes by enzyme-catalyzed chemical modification. Advanc. Enzymol. 32, 297–326 (1969).
Kelly, M.: Some properties of purified nitrogenase of Azotobacter chroococcum. Biochim. biophys. Acta (Amst.) 171, 9–15 (1969a).
—: Comparisons and cross reactions of nitrogenase from Klebsiella pneumoniae, Azotobacter chroococcum and Bacillus polymixa. Biochim. biophys. Acta (Amst.) 191, 527–534 (1969b).
Knight, E., Hardy, R. W. F.: Isolation and characteristics of flavodoxin from nitrogen-fixing Clostridium pasteurianum. J. biol. Chem. 241, 2752–2756 (1966).
——: Flavodoxin: chemical and biological properties. J. biol. Chem. 242, 1370–1374 (1967).
Krogman, D. W., Jagendorf, A. I., Avron, M.: Uncouplers of spinach chloroplast photosynthetic phosphorylation. Plant. Physiol. 34, 272–277 (1959).
Oppenheim, J., Fisher, R. J., Wilson, P. W., Marcus, L.: Properties of a soluble nitrogenase in Azotobacter. J. Bact. 101, 292–296 (1970).
Parker, C. A., Scutt, P. B.: The effect of oxygen on nitrogen fixation by Azotobacter. Biochim. biophys. Acta (Amst.) 38, 230–238 (1960).
Patil, R. B., Pengra, R. M., Yoch, D. C.: Effects of nitrogen supplements on nitrogen fixation by Aerobacter aerogenes. Biochim. biophys. Acta (Amst.) 136, 1–5 (1967).
Pengra, R. M., Wilson, P. W.: Physiology of nitrogen fixation by Aerobacter aerogenes. J. Bact. 75, 21–25 (1958).
Richmond, M. H.: The effect of amino acid analogous on growth and protein synthesis in bacteria. Bact. Rev. 26, 398–420 (1962).
Simon, R. D.: Cyanophycin granules from the blue-green alga Anabaena cylindrica: a reserve material consisting of copolymers of aspartic acid and arginine. Proc. nat. Acad. Sci. (Wash.) 68, 265–267 (1971).
Sirenko, L. A., Stezenko, N. M., Arendarchuk, V. V., Kusmenko, M. I.: The role of oxygen in the activity of some blue-green algae. Mikrobiologiya 37, 239–244 (1968).
Smillie, R. M.: Isolation of two proteins with chloroplast ferredoxin activity from a blue-green alga. Biochem. biophys. Res. Commun. 20, 621–629 (1965).
Sorger, G. J.: Regulation of nitrogen fixation in Azotobacter vinelandii OP and in an apparently partially constitutive mutant. J. Bact. 95, 1721–1726 (1968).
Stewart, W. D. P., Pearson, H. W.: Effects of aerobic and anaerobic conditions on growth and metabolism of blue-green algae. Proc. roy. Soc. B 175, 293–311 (1970).
Strandberg, G. W., Wilson, P. W.: Formation of the nitrogen-fixing enzyme system in Azotobacter vinelandii. Canad. J. Microbiol. 14, 25–31 (1968).
Vazquez, D.: Mode of action of chloramphenicol and related antibiotics. In: Biochemical studies on antimicrobial drugs. Eds. B. A. Newton and P. E. Reynolds. Sixteenth symposium of the society for general microbiology, pp. 169–191. London: Cambridge University Press 1966.
Walker, J. B.: Canavanine and homoarginine as antimetabolites of arginine and lysine in yeast and algae. J. biol. Chem. 212, 207–215 (1955).
Yarmolinsky, M., De La Haba, G.: Inhibition by puromycin of amino acid incorporation into protein. Proc. nat. Acad. Sci. (Wash.) 45, 1721–1729 (1959).
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Bone, D.H. Kinetics of synthesis of nitrogenase in batch and continuous culture of Anabaena flos-aquae . Archiv. Mikrobiol. 80, 242–251 (1971). https://doi.org/10.1007/BF00410125
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DOI: https://doi.org/10.1007/BF00410125