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Der Nachweis verschiedener Kollagentypen im Rinderauge

The proof of different types of collagen in the bovine eye

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Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Aims and scope Submit manuscript

Summary

Characteristically stained polyacrylamide gels can be obtained by disk-electrophoresis in acid medium of several tissues of the bovine eye. The technique permits to prove different types of collagen in the eye, and allows the differentiation and identification of the tissues. By these results the different tissues of the eye can be divided into three groups.

  1. 1.

    Tissues showing two α collagen components in polyacrylamide gel (cornea, sclera, iris, ciliary body, anterior lens capsule, and the pigmented epithelium of the retina).

  2. 2.

    Tissues possessing one α component only (zonula fiber and vitreous body).

  3. 3.

    Tissues which show neither the α nor the β and γ component of collagen (lens nucleous and retina without pigmented epithelium).

Zusammenfassung

Mit Hilfe der Disk-Elektrophorese im sauren Medium von verschiedenen Geweben des Rinderauges können charakteristisch gefärbte Polyacrylamidgele erhalten werden. Diese Technik erlaubt den Nachweis verschiedener Kollagentypen im Rinderauge sowie die Differenzierung und Identifizierung dieser Gewebe. Die Ergebnisse ermöglichen eine Einteilung der verschiedenen Gewebe in 3 Gruppen.

  1. 1.

    Gewebe, die im Polyacrylamidgel zwei α-Kollagenkomponenten zeigen (Hornhaut, Lederhaut, Regenbogenhaut, Ciliarkörper, vordere Linsenkapsel und das Pigmentepithel der Netzhaut).

  2. 2.

    Gewebe, die nur eine α-Komponente aufweisen (Zonulafaser und Glaskörper).

  3. 3.

    Gewebe, die weder αnoch die β- und γ-Kollagenkomponente zeigen (Linsenkern und Netzhaut ohne Pigmentepithel).

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Schmut, O., Reich, M.E. & Zirm, M. Der Nachweis verschiedener Kollagentypen im Rinderauge. Albrecht von Graefes Arch. Klin. Ophthalmol. 196, 71–77 (1975). https://doi.org/10.1007/BF00410028

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  • DOI: https://doi.org/10.1007/BF00410028

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