Summary
A radiorespirometric technique was used to determine the effects of sodium N-methyldithiocarbamate (NaMDC) on uptake and oxidation of glucose by Fusarium oxysporum f. sp. lycopersici. The principal glucose oxidation pathway of the fungus was found to be the pentose cycle. Shifts to other oxidation pathways were not significant even when the fungus was treated with extremely high concentrations of NaMDC (5×10-2 M). Only when zinc ions were added to NaMDC solutions were shifts in pathway (and a drastic increase in toxicity) noted. The main effect of NaMDC was to delay the complete oxidation of glucose to CO2. In the concentration range between 0.5 to 10.0×10-2 M NaMDC dose response curves were bimodal with respect to spore germination, colony development, and CO2 production; with respect to glucose uptake the curves were unimodal. The bimodal dosage response curves could be divided into a first zone of inhibition, a zone of reversed toxicity, and a second zone of inhibition. Within the first zone of inhibition, colony development proved to be more sensitive to NaMDC than spore germination. In the zone of reversed toxicity the incorporation of carbon into the spores drops sharply, while in the second zone of inhibition a strong inhibition of glucose uptake is the dominating effect. It is indicated that NaMDC interfers with biosynthetic activities rather than enzymes of catabolic pathways.
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Abbreviations
- NaMDC:
-
Sodium N-methyldithiocarbamate
- NaDMDC:
-
Sodium dimethyldithiocarbamate
- TMTD:
-
Tetramethylthiuram disulfide, [=Bis(dimethylthiocarbamyl)disulfid, Thiram®]
- DMTD:
-
N,N′-dimethylthiuram disulfide [=Bis-(methylthiocarbamyl)disulfid]
- MIT:
-
Methylisothiocyanate
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Domsch, K.H., Corden, M.E. Investigations on the mode of action of sodium N-methyldithiocarbamate in Fusarium oxysporum f. sp. lycopersici . Archiv. Mikrobiol. 88, 353–363 (1973). https://doi.org/10.1007/BF00409946
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DOI: https://doi.org/10.1007/BF00409946