Summary
The metabolism and fate of specifically labeled glucose-14C were compared to mannitol-l-14C and arabitol-l-14C during basidiospore germination of Schizophyllum commune on glucose-asparagine minimal broth. Glucose-l-14C metabolism led to more 14CO2 evolution than glucose-6-14C in spores and the former activity increased upon germination. Liberation of 14CO2 from glucose-3,4-14C increased at 8 h to 12 h of germination and exceeded the amount of radioactive 14CO2 released from glucose-1-14C. The 14CO2 released from glucose-2-14C increased continually during germination while only minor changes in 14CO2 evolution occurred with glucose-6-14C. Unlabeled ethanol (0.25 M) inhibited 14CO2 evolution with glucose-3,4-14C and ungerminated spores and this inhibition disappeared upon germination.
More 14CO2 was evolved from labeled glucose during germination and less radioactivity became associated with cellular material. Of the latter, alcohol-soluble extracts of spores or germlings contained mainly radioactive trehalose, less mannitol and little or no labeled arabitol, and this decreased upon germination. Germlings also converted more radioactive glucose-14C into KOH-insoluble material and KOH-soluble components. Spores or germlings converted arabitol-1-14C primarily into trehalose and this was not the case for mannitol-1-14C.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aitken, W. B., Niederpruem, D. J.: Ultrastructural changes and biochemical events in basidiospore germination of Schizophyllum commune. J. Bact. 104, 981–988 (1970).
Aitken, W. B., Niederpruem, D. J.: Isotopic studies of carbohydrate metabolism during basidiospore germination in Schizophyllum commune. I. Uptake of radioactive glucose and sugar alcohols. Arch. Mikrobiol. 82, 173–183 (1972).
Baumann, P., Wright, B. E.: The phosphofructokinase of Dictyostelium discoideum. Biochemistry 7, 3653–3661 (1968).
Baumann, P., Wright, B. D.: The fructose 1,6-diphosphatase of Dictyostelium discoideum. Biochemistry 8, 1655–1659 (1969).
Braun, M. L., Niederpruem, D. J.: Erythritol metabolism in wild-type and mutant strains of Schizophyllum commune. J. Bact. 100, 625–634 (1969).
Budd, K., Sussman, A. S., Eilers, F. I.: Glucose-C14 metabolism of dormant and activated ascospores of Neurospora. J. Bact. 91, 551–561 (1966).
Cleland, S. V., Coe, E. L.: Activities of glycolytic enzymes during the early stages of differentiation in the cellular slime mold Dictyostelium discoideum. Biochim. biophys. Acta (Amst.) 156, 44–50 (1968).
Cochrane, V. W.: Respiration and spore germination. In: M. F. Madelin (ed.), The fungus spore, pp. 201–215. proc. 18th Symp. Colston Res. Soc. London: Butterworth Sci. Publ. 1966.
Cochrane, V. W., Berry, S. J., Simon, F. G., Cochrane, J. C., Collins, C. B., Levy, J. A., Holmes, P. K.: Spore germination and carbon metabolism in Fusarium solani. III. Carbohydrate respiration in relation to germination. Plant Physiol. 38, 533–541 (1963).
Cochrane, V. W., Cochrane, J. C.: Spore germination and carbon metabolism in Fusarium solani. V. Changes in anaerobic metabolism and related enzyme activities during development. Plant Physiol. 41, 810–814 (1966).
Cotter, D. A., Niederpruem, D. J.: Control of arabitol formation in Schizophyllum commune development. Arch. Mikrobiol. 78, 128–138 (1971).
Eilers, F. I., Ikuma, H., Sussman, A. S.: Changes in metabolic intermediates during activation of Neurospora ascospores. Canad. J. Microbiol. 16, 1351–1356 (1970).
Garraway, M. O., Weinhold, A. R.: Influence of ethanol on the distribution of glucose-C14 assimilated by Armillaria mellea. Phytopathology 58, 1652–1657 (1968).
Isenberg, P., Niederpruem, D. J.: Control of erythritol dehydrogenase in Schizophyllum commune. Arch. Mikrobiol. 56, 22–30 (1967).
Niederpruem, D. J., Dennen, D. W.: Kinetics, nutrition and inhibitor properties of basidiospore germination in Schizophyllum commune. Arch. Mikrobiol. 54, 91–105 (1966).
Niederpruem, D. J., Hafiz, A., Henry, L.: Polyol metabolism in the Basidiomycete Schizophyllum commune. J. Bact. 89, 954–959 (1965a).
Niederpruem, D. J., Hunt, S.: Polyols in Schizophyllum commune. Amer. J. Bot. 54, 241–245 (1967).
Niederpruem, D. J., Jersild, R. A.: Cellular aspects of morphogenesis in the mushroom Schizophyllum commune. CRC Crit. Rev. Microbiol. 1, 545–576 (1972).
Niederpruem, D. J., Updike, J., Henry, L.: Hexose monophosphate metabolism during basidiospore germination in Schizophyllum commune. J. Bact. 89, 908–909 (1965b).
Weinhold, A. R.: Rhizomorph production by Armillaria mellea induced by ethanol and related compounds. Science 142, 1065–1066 (1963).
Weinhold, A. R., Garraway, M. O.: Nitrogen and carbon nutrition of Armillaria mellea in relation to growth-promoting effects of ethanol. Phytopathology 56, 108–112 (1966).
Wright, B. E., Bloom, B.: In vivo evidence for metabolic shifts in the differentiating slime mold. Biochim. biophys. Acta (Amst.) 48, 342–346 (1961).
Wright, B. E., Bruhmuller, M.: The effect of exogenous glucose concentration of C-6/C-1 ratio. Biochim. biophys. Acta (Amst.) 82, 203–204 (1964).
Wright, B. E., Bruhmuller, M., Ward, C.: Studies in vivo on hexose metabolism in Dictyostelium discoideum. Develop. Biol. 9, 287–297 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Aitken, W.B., Niederpruem, D.J. Isotopic studies of carbohydrate metabolism during basidiospore germination in Schizophyllum commune . Archiv. Mikrobiol. 88, 331–344 (1973). https://doi.org/10.1007/BF00409944
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00409944