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Studies on 1-methyl adenine transfer RNA methyltransferase of Mycobacterium smegmatis

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Abstract

The presence of 1-methyl adenine in transfer RNA is a feature that Mycobacterium smegmatis shares with only a few other prokaryotes. The enzyme 1-methyl adenine tRNA methyl transferase from this source has been purified and the preliminary results show the presence of two activity peaks with different substrate specificity.

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Abbreviations

tRNA:

Transfer Ribonucleic acid

EDC:

1-ethyl-3(3-dimethyl)amino Propyl Carbodiimide

PCA:

Perchloric acid

SAH:

S-adenosyl-homocystine

SIBA:

5′-deoxy-5′-S-isobutylthioadenosine

SAM:

S-adenosyl methionine

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Authors and Affiliations

  1. Microbiology and Cell Biology Laboratory, Indian Institute of Science, 560012, Bangalore, India

    Vani Brahmachari & T. Ramakrishnan

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  1. Vani Brahmachari
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  2. T. Ramakrishnan
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Brahmachari, V., Ramakrishnan, T. Studies on 1-methyl adenine transfer RNA methyltransferase of Mycobacterium smegmatis . Arch. Microbiol. 140, 91–95 (1984). https://doi.org/10.1007/BF00409778

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  • DOI: https://doi.org/10.1007/BF00409778

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