Abstract
The broad-spectrum herbicide glyphosate inhibits the growth of Candida maltosa and causes the accumulation of shikimic acid and shikimate-3-phosphate. Glyphosate is a potent inhibitor of three enzymes of aromatic amino acid biosynthesis in this yeast. In relation to tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase and dehydroquinate synthase, the inhibitory effect appears at concentrations in the mM range, but 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase is inhibited by micromolar concentrations of glyphosate. Inhibition of partially purified EPSP synthase reaction by glyphosate is competitive with respect to phosphoenolpyruvate (PEP) with a K i -value of 12 μM. The app. K m for PEP is about 5-fold higher and was 62 μM. Furthermore, the presence of glyphosate leads to derepression of many amino acid biosynthetic enzymes.
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Abbreviations
- DAHP:
-
3-deoxy-D-arabino-heptulosonate 7-phosphate
- EPSP synthase:
-
5-enolpyruvylshikimate 3-phosphate synthase
- PEP:
-
phosphoenolpyruvate
- S-3-P:
-
shikimate-3-phosphate
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Bode, R., Melo, C. & Birnbaum, D. Mode of action of glyphosate in Candida maltosa . Arch. Microbiol. 140, 83–85 (1984). https://doi.org/10.1007/BF00409776
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DOI: https://doi.org/10.1007/BF00409776