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Mutants of Pseudomonas aeruginosa unable to inactivate allantoinase and NADP-dependent glutamate dehydrogenase

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Abstract

Both allantoinase and NADP-GDH in Pseudomonas aeruginosa were inactivated when cells reached the stationary phase of growth. Mutants unable to inactivate these enzymes were isolated. Results with recombinants showed that the mutation is not located in the structural genes of these enzymes but in an independent gene involved in the inactivation.

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Abbreviations

NADP-GDH:

NADP-dependent glutamate dehydrogenase

Ani- mutant:

allantoinase non-inactivating mutant

GOGAT:

glutamate synthase

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, University of Nijmegen, Toernooiveld, NL-6525 ED, Nijmegen, The Netherlands

    R. A. M. M. Smits, W. M. H. van de Wijngaard, A. P. M. Stassen & C. van der Drift

Authors
  1. R. A. M. M. Smits
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  2. W. M. H. van de Wijngaard
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  3. A. P. M. Stassen
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  4. C. van der Drift
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Smits, R.A.M.M., van de Wijngaard, W.M.H., Stassen, A.P.M. et al. Mutants of Pseudomonas aeruginosa unable to inactivate allantoinase and NADP-dependent glutamate dehydrogenase. Arch. Microbiol. 140, 40–43 (1984). https://doi.org/10.1007/BF00409769

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  • DOI: https://doi.org/10.1007/BF00409769

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