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Der Redoxzustand des NAD(P) und der Cytochrome b und c2 in Abhängigkeit vom pO2 bei einigen Athiorhodaceae

The influence of the pO2 on the redox balance of NAD(P) and of cytochrome b and c2 in some Athiorhodaceae

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Summary

If the oxygen partial pressure in growing cultures of Rhodospirillum rubrum, Rhodopseudomonas spheroides, and Rhodopseudomonas capsulata is slowly lowered the level of reduced nicotinamide-adenine dinucleotide is strongly increased but not before the pO2 is dropped beneath 0.2–0.5 mm Hg.

A very quick change from aerobic to strict anaerobic conditions in a dense suspension of R. rubrum causes a significant overreduction of the nicotinamide-adenine dinucleotides. After switching on the aeration an overoxidation is observable.

The reduction of cytochromes b and c2 obeys the same kinetics as the reduction of NAD(P)+. A sudden aeration is followed by an overoxidation, which continues for more than 1 min.

The changes in the ratio of the reduced to the oxidised states of the nicotinamide-adenine dinucleotide and the cytochromes are correlated to the breakdown of the metabolism (oxidative phosphorylation). But the synthesis of bacteriochlorophyll is induced by a decrease of oxygen partial pressure down to 50- mm Hg (depended from the organism). It has been shown in these experiments that the induction of the synthesis of bacteriochlorophyll in the dark by changing the pO2 is not directly correlated to the change in the redox state of NAD(P)+/NAD(P)H and of the cytochromes b and c2.

Zusammenfassung

Der Gehalt an reduzierten Nicotinamid-adenin-dinucleotiden bei Rhodospirillum rubrum, Rhodopseudomonas spheroides und Rps. capsulata wird beim Absenken des pO2 erst zwischen 0,2 und 0,5 mm Hg deutlich erhöht.

Beim schnellen Wechsel (dichte Bakteriensuspension) zur Anaerobiose tritt bei R. rubrum eine deutliche Überreduktion der Nicotinamid-adenin-dinucleotide ein und bei erneuter Belüftung eine Überoxydation.

Der Reduktionszustand der Cytochrome b+c2 und c2 (Differenzspektren 422 mμ bzw. 426 mμ oder 428 mμ minus 405 mμ und 550 mμ minus 545 mμ bzw. 550 mμ minus 560 mμ) erhöht sich etwa zur gleichen Zeit wie die Konzentration von NAD(P)H. Bei erneuter Belüftung tritt immer eine Überoxydation ein, die über 1 min andauern kann.

Die änderung des Redoxgleichgewichts von NAD(P)+/NAD(P)H und der Cytochrome ist korreliert mit dem Zusammenbruch des Energiestoffwechels (oxydative Phosphorylierung), zeigt aber keine Beziehung zum Beginn der Bakteriochlorophyll-synthese unter semiaeroben Wachstumsbedingungen, die schon bei höherem pO2 erfolgt.

Damit konnte gezeigt werden, daß die Induktion der BChl.-Synthese in Dunkelkulturen durch Änderung des O2-Partialdruckes in keinem direkten Zusammenhang zur Änderung des Redoxgleichgewichtes von NAD(P)+/NAD(P)H und der Cytochrome b und c2 steht.

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Abbreviations

NAD(P):

Nicotinamid-adenin-dinucleotid + Nicotinamid-adenin-dinucleotid-phosphat

pO2 :

Sauerstoffpartialdruck

BChl.:

Bacteriochlorphyll

Literatur

  • Bartsch, R. G.: Spectroscopic properties of purified cytochromes of photosynthetic bacteria. In: Bacterial photosynthesis, pp. 475–494. Ed. H. Gest, A. San Pietro, and L. P. Vernon. Yellow Springs: The Antioch Press 1963.

    Google Scholar 

  • Biedermann, M., G. Drews, R. Marx u. J. Schröder: Der Einfluß des Sauerstoffpartialdruckes und der Antibiotica Actinomycin und Puromycin auf das Wachstum, die Synthese von Bacteriochlorophyll und die Thylakoidmorphogenese in Dunkelkulturen von Rhodospirillum rubrum. Arch. Mikrobiol. 56, 133–147 (1967).

    Google Scholar 

  • Chance, B., and J. Olson: Primary metabolic events associated with photosynthesis. Arch. Biochem. 88, 54–58 (1960).

    Google Scholar 

  • Cohen-Bazire, G., W. R. Sistrom, and R. Y. Stanier: Kinetic studies of pigment synthesis by nonsulfur purple bacteria. J. cell. comp. Physiol. 49, 25–35 (1957).

    Google Scholar 

  • Drews, G.: Untersuchungen zur Regulation der Bacteriochlorophyllsynthese bei Rhodospirillum rubrum. Arch. Mikrobiol. 51, 196–198 (1965).

    Google Scholar 

  • —: Zur Regulation der Tetrapyrrolsynthese bei Rhodospirillum rubrum. Z. Naturforsch. 21 b, 1224–1229 (1966).

    Google Scholar 

  • — u. G. Schön: Zur Regulation der Synthese des Bakterienphotosyntheseapparates. Zbl. Bakt., I. Abt. Orig. 205, 38–41 (1967).

    Google Scholar 

  • Duysens, L. N. M., and J. Amesz: Fluorescence spectrophotometry of reduced phosphopyridine nucleotide in intact cells in the near-ultraviolet and visible region. Biochim. biophys. Acta (Amst.) 24, 19–26 (1957a).

    Google Scholar 

  • —, and G. Sweep: Fluorescence spectrophotometry of pyridine nucleotide in photosynthesizing cells. Biochim. biophys. Acta (Amst.) 25, 13–16 (1957b).

    Google Scholar 

  • Horio, T., and M. D. Kamen: Some haem protein-linked pyridine nucleotide oxidation systems in Rhodospirillum rubrum. Biochim. biophys. Acta (Amst.) 43, 382–392 (1960).

    Google Scholar 

  • ——: Optimal oxidation-reduction potentials and endogenous co-factors in bacterial photophosphorylation. Biochemistry 1, 144–153 (1962).

    Google Scholar 

  • Ke, B., and E. Ngo: Light-induced rapid absorption changes during photosynthesis. VIII. Cytochrome and bacteriochlorophyll reactions in Rhodospirillum rubrum cells. Biochim. biophys. Acta (Amst.) 143, 319–331 (1967).

    Google Scholar 

  • Olson, J. M., L. N. M. Duysens, and G. H. M. Kronenberg: Spectrofluorometry of pyridine nucleotide reactions in Chromatium. Biochim. biophys. Acta (Amst.) 36, 125–131 (1959).

    Google Scholar 

  • Porra, R. J., and J. Lascelles: Haemoproteins and Haem synthesis in facultative photosynthetic and denitrifying bacteria. Biochem. J. 94, 120–126 (1965).

    Google Scholar 

  • Schön, G.: Der Einfluß von Amytal, 2,4-Dinitrophenol und Phenazinmethosulfat auf die Bacteriochlorophyllsynthese in anaeroben Kulturen von Rhodospirillum rubrum. Arch. Mikrobiol. 61, 187–194 (1968).

    Google Scholar 

  • Sistrom, W. R.: The kinetics of the synthesis of photopigments in Rhodopseudomonas spheroides. J. gen. Microbiol. 28, 607–616 (1962).

    Google Scholar 

  • —: A note on the effect of inhibitors of electron transport and phosphorylation on photopigment synthesis in Rhodopseudomonas spheroides: In: Bacterial photosynthesis, pp. 53–60. Ed. H. Gest, A. San Pietro, and L. P. Vernon. Yellow Springs: The Antioch Press 1963.

    Google Scholar 

  • Smith, L.: Reactions of Rhodospirillum rubrum extracts with cytochrom c and cytochrom c2. J. biol. Chem. 234, 1571–1574 (1959a).

    Google Scholar 

  • —: Respiration and light-induced phosphorylation in extracts of Rhodospirillum rubrum. J. biol. Chem. 234, 1575–1579 (1959b).

    Google Scholar 

  • Yamashita, J., S. Yoshimura, Y. Matuo, and T. Horio: Relationship between photosynthetic and oxidative phosphorylations in chromatophores from light grown cells of Rhodospirillum rubrum. Biochim. biophys. Acta (Amst.) 143, 154–172 (1967).

    Google Scholar 

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  1. Lehrstuhl für Mikrobiologie der Universität Freiburg i. Br., Freiburg i. Br., Deutschland

    G. Schön & G. Drews

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  1. G. Schön
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  2. G. Drews
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Schön, G., Drews, G. Der Redoxzustand des NAD(P) und der Cytochrome b und c2 in Abhängigkeit vom pO2 bei einigen Athiorhodaceae . Archiv. Mikrobiol. 61, 317–326 (1968). https://doi.org/10.1007/BF00409668

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