Summary
-
1.
The higher fatty acids of Achromobacter were identified by gas chromatography, NMR and mass spectrometry as capric, lauric, myristic, 9,10-hexadecenoic, 11,12-octadecenoic, β-hydroxy-myristic and cis-9,10-methylenehexadecanoic acid.
-
2.
The composition of the fatty acids is in agreement with the taxonomic classification of Achromobacter. Thus one finds characteristic differences and correspondences with other families of the order of Eubacteriales.
Zusammenfassung
-
1.
Die in Achromobacter enthaltenen höheren Fettsäuren wurden mit Hilfe der Gaschromatographie, Kernresonanz- und Massenspektroskopie als Caprin-, Laurin-, Myristin-, Palmitin-, 9,10-Hexadecen-, 11,12-Octadecen-, β-Hydroxymyristin- und cis-9,10-Methylen-Hexadecansäure identifiziert.
-
2.
Die taxonomische Stellung von Achromobacter spiegelt sich in seinem Fettsäurespektrum wider, das sowohl charakteristische Unterschiede wie Übereinstimmungen mit anderen Familien der Ordnung Eubacteriales aufweist.
Similar content being viewed by others
Abbreviations
- PEGS:
-
Polyäthylenglykolsuccinat.
- NPGS:
-
Neopentylglykolsuccinatpolyester.
Literatur
Bishop, D. G., Still, J. L.: Fatty acid metabolism in Serratia marcescens. II. The occurrence of hydroxy acids. Biochem. biophys. Res. Commun. 7, 337–341 (1962).
Burchfield, H. P., Storrs, E. E.: in: Biochemical applications of gas chromatography, p. 497. New York-London: Academic Press 1962.
Chang, Ta-Chuang Lo, Sweeley, C. C.: Studies on canine adrenal polyenoic acids: locating double bonds by periodate-permanganate oxidation and gas-liquid chromatography. J. Lipid. Res. 3, 170–176 (1962).
Christie, W. W., Gunstone, F. D., Ismail, I. A., Wade, L.: Fatty acids part 17. The synthesis and chromatographic and spectroscopic properties of the cyclopropane esters derived from all the methyl octadecenoates (Δ2 − Δ17). Chem. phys. Lipids 2, 196–202 (1968).
Himes, R. H., Lynen, F.: Metabolism of acetoacetate by Achromobacter IV S. Biochem. Z. 340, 206–212 (1968).
Hofmann, K., Lucas, R. A., Sax, S. M.: The chemical nature of the fatty acids of Lactobacillus arabinosus. J. biol. Chem. 195, 473–485 (1952).
— Sax, S. M.: The chemical nature of the fatty acids of Lactobacillus casei. J. biol. Chem. 205, 55–63 (1953).
— Tausig, F.: On the identity of phytomonic and lactobacillic acids. A reinvestigation of the fatty acid spectrum of Agrobacterium (Phytomonas) tumefaciens. J. biol. Chem. 213, 415–432 (1955 a).
—— The chemical nature of the fatty acids of a group C Streptococcus speices. J. biol. Chem. 213, 415–423 (1955 b).
Ikawa, M., Koepfli, J. B., Mudd, S. G., Niemann, C.: An agent from E. coli causing hemorrhage and regression of an experimental mouse tumor. III. The component fatty acids of the phospholipide moiety. J. Amer. Chem. Soc. 75, 1035–1038 (1953).
Jarvis, F. G., Johnson, M. J.: Glyco-lipide produced by Pseudomonas aeruginosa. J. Amer. chem. Soc. 71, 4124–4126 (1949).
Jauch, R., Lynen, F.: unveröffentlicht.
Kaneshiro, T., Marr, A. G.: Cis-9,10-methylene hexadecanoic acid from the phospholipids of Escherichia coli. J. biol. Chem. 236, 2615–2619 (1961).
—— Hydroxy fatty acids of Azotobacter agilis. Biochim. Biophys. Acta 70, 271–277 (1963).
Kates, M.: Bacterial lipids. In: Advances in Lipid Research, hrsg. von R. Paoletti u. D. Kritchevsky, Vol. 2, p. 17 ff. New York-London: Academic Press 1964.
Knappe, J., Schlegel, H., Lynen, F.: Zur biochemischen Funktion des Biotins I. Die Beteiligung der β-Methylcrotonyl-Carboxylase an der Bildung von β-Hydroxy-β-methylglutaryl-CoA aus β-Hydroxy-isovalery-CoA. Biochem. Z. 335, 101–122 (1961).
Law, J. H., Zalkin, H., Kaneshiro, T.: Transmethylation reactions in bacterial lipids. Biochim. biophys. Acta (Amst.) 70, 143–153 (1963).
Lederer, E.: The origin and function of some methyl groups in branched-chain fatty acids, plant sterols and quinones. Biochem. J. 93, 449–468 (1964).
Le Goff, E.: Cyclopropanes from an easily prepared, highly active zinc-cupper couple, dibrommethane, and olefins. J. org. Chem. 29, 2048–2050 (1964).
O'Leary, W. M.: S-Adenosylmethionine in the biosynthesis of bacterial fatty acids. J. Bact. 84, 967–972 (1962).
Ryhage, R., Stenhagen, E.: Mass spectrometry of long-chain esters. In: Mass spectrometry of organic ions, hrsg. von Mc. Lafferty p. 399 ff. New York-London: Academic Press 1963.
Westphal, O., Lüderitz, O.: Chemische Erforschung von Lipopolysacchariden gramnegativer Bakterien. Angew. Chem. 66, 407–417 (1954).
Zalkin, H., Law, J. H., Goldfine, H.: Enzymatic synthesis of cyclopropane fatty acids catalyzed by bacterial extracts. J. biol. Chem. 238, 1242–1248 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jauch, R., Schiele, U. & Lynen, F. Die höheren Fettsäuren in Achromobacter . Archiv. Mikrobiol. 72, 186–198 (1970). https://doi.org/10.1007/BF00409524
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00409524