Summary
Freeze-etch replicas of basidiospores of Panaeolus campanulatus treated for 16 hours in glycerol showed that germination processes had been initiated. The plasmalemma bore evaginations, abstricting vesicles into the paramural space, and the nuclear envelope evaginated abstricting promitochondrial initials. The significance of these results is discussed in relation to previous work on chemically-fixed spores of various fungi.
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References
Campbell, R.: An electron microscopic study of spore structure and development in Alternaria brassicola. J. gen. Microbiol. 54, 381–392 (1969).
Frey-Wyssling, A., Muhlethaler, K.: Ultrastructural plant cytology, p. 228. Amsterdam-London-New York: Elsevier Pub. Co. 1965.
Griffiths, D. A.: Paramural bodies in hyphae of Verticillium dahliae Kleb. revealed by freeze-etching. Arch. Mikrobiol. 73, 331–336 (1970).
Hawker, Lilian E., Abbott, P. McV.: An electron microscope study of maturation and germination of sporangiospores of two species of Rhizopus. J. gen. Microbiol. 32, 295–298 (1963).
—, Hendy, R. J.: An electron microscope study of germination of conidia of Botrytis cinerea. J. gen. Microbiol. 33, 43–46 (1963).
Hyde, J. M., Wilkinshaw, C. H.: Ultrastructure of basidiospores and mycelium of Lenzites saepiaria. J. Bact. 92, 1218–1227 (1966).
Lowry, R. J., Sussmann, A. S.: Ultrastructural changes during germination of ascospores of Neurospora tetrasperma. J. gen. Microbiol. 51, 403–409 (1968).
Mayor, H. D., Hampton, J. C., Rosario, B.: A simple method for removing the resin from epoxy-embedded tissue. J. Cell Biol. 9, 909 (1961).
Moor, H.: Die Gefrier-Fixation lebender Zellen und ihre Anwendung in der Elektronenmikroskopie. Z. Zellforsch. 62, 546–580 (1964).
—: The performance of freeze-etching and the interpretation of results concerning the surface structure of membranes and the fine structure of microtubules and spindle fibres. Balzers High Vacuum Report No. 9. Balzers AG., Balzers. Principallity of Liechtenstein (1966).
Sassen, M. M. A., Remsen, C. C., Hess, W. H.: Fine structure of Penicillium megasporum conidiospores. Protoplasma (Wien) 64, 75–88 (1967).
Stocks, D. L., Hess, W. M.: Ultrastructure of dormant and germinated basidiospores of a species of Psilocybe. Mycologia 62, 176–191 (1970).
Sussman, A. S.: Dormancy and spore germination. In The Fungi. Eds. Ainsworth and Sussman, Vol. III. New York-London: Academic Press 1966.
Voelz, H., Niederpruen, K. J.: Fine structure of basidiospores of Schizophyllum commune. J. Bact. 88, 1497–1502 (1964).
Wells, K.: The basidia of Exidia nucleata. II. Development. Amer. J. Bot. 51, 360–370 (1964).
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Griffiths, D.A. The fine structure of basidiospores of Panaeolus campanulatus (L.) Fr. revealed by freeze-etching. Archiv. Mikrobiol. 76, 74–82 (1970). https://doi.org/10.1007/BF00409316
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DOI: https://doi.org/10.1007/BF00409316