Summary
The role of adenine nucleotides in the control of the energy metabolism of Chromatium has been studied through the measurement of the levels of ATP ADP, and AMP in growing cultures. Comparison of these levels with the cellular chlorophyll levels indicates that ATP concentration, and not those of ADP, AMP, or any function of concentration of these three nucleotides, is a controlling factor in chlorophyll synthesis. In addition, the relationship of cellular ATP and chlorophyll levels to sulfur metabolism furnishes further evidence for the existence of two photosystems, postulated previously. The effects of high levels of exogenous ATP and ADP are consistent with these findings.
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References
Atkinson, D. E.: The interaction of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochemistry 7, 4030–4034 (1968).
— Walton, G. M.: Adenosine triphosphate conservation in metabolic regulation. J. biol. Chem. 242, 3239–3241 (1967).
—: Regulation of enzyme function. Ann. Rev. Microbiol. 23, 47–68 (1969).
Bomsel, J.-L., Pradet, A.: Study of adenosine 5′-mono-, di-, and triphosphates in plant tissues. IV. Regulation of the level of nucleotides, in vivo, by adenylate kinase: theoretical and experimental study. Biochim. biophys. Acta (Amst.) 162, 230–242 (1968).
Bücher, T., Pfleiderer, G.: Pyruvate kinase from muscle. In: Methods in Enzymology, Vol. I, pp. 435–440. Eds.: S. P. Colowick and N. O. Kaplan. New York: Academic Press 1955.
Cohen-Bazire, G., Sistrom, W. R., Stanier, R. Y.: Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J. cell comp. Physiol. 49, 25–68 (1957).
Cole, H. A., Wimpenny, J. W. T., Hughes, D. E.: The ATP pool in Escherichia coli. Biochim. biophys. Acta (Amst.) 143, 445–453 (1967).
Cusanovich, M. A., Bartsch, R. G., Kamen, M. D.: Light-induced electron transport in Chromatium Strain D. II. Light-induced absorbance changes in Chromatium chromatophores. Biochem. biophys. Acta (Amst.) 153, 397–417 (1968).
Fanica-Gaignier, M., Clement-Metral, J., Kamen, M. D.: Adenine nucleotide levels in growing cultures of Rhodopseudomonas spheroides. Biochim. biophys. Acta (in press) (1971).
Forrest, W. W., Walker, D. J.: Synthesis of reserve materials for endogenous metabolism in Streptococcus faecalis. J. Bact. 89, 1448–1452 (1965).
Gajdos, A., Gajdos-Török, M.: The quantitative regulation of the biosynthesis of porphyrins by intracellular ATP concentration. Biochem. Med. 2, 372–388 (1969).
Gemerden, H., van: On the ATP generation by Chromatium in darkness. Arch. Mikrobiol. 64, 118–124 (1968).
Gibson, J., Morita, S.: Changes in adenine nucleotides of intact Chromatium D produced by illumination. J. Bact. 93, 1544–1550 (1967).
Hageage, G. J., Jr., Eanes, E. D., Gherna, R. L.: X-ray diffraction studies of the sulfur globules accumulated by Chromatium species. J. Bact. 101, 464–469 (1970).
Hind, G., Olson, J. M.: Electron transport pathways in photosynthesis. Ann. Rev. Plant. Physiol. 19, 249–282 (1968).
Holm-Hansen, O., Booth, C. R.: The measurement of adenosine triphosphate in the ocean and its ecological significance. Limnology and Oceanography 11, 510–519 (1966).
Morita, S.: Evidence for three photochemical systems in Chromatium D. Biochim. biophys. Acta (Amst.) 153, 241–247 (1968).
Pradet, A.: Étude des adenosine-5′-mono-, di- et tri-phosphates dans les tissues végétaux. I. Dosage enzymatique. Physiol. vég. 5, 209–221 (1967).
Ramirez, J., Smith, L.: Synthesis of adenosine triphosphate in intact cells of Rhodospirillum rubrum and Rhodopseudomonas spheroides on oxygenation or illumination. Biochim. biophys. Acta (Amst.) 153, 466–475 (1968).
Schmidt, G. L., Kamen, M. D.: Variable cellular composition of Chromatium in growing cultures. Arch. Mikrobiol. 73, 1–18 (1970).
Schön, G.: Der Einfluß der Kulturbedingungen auf den ATP- ADP-, und AMP-Spiegel bei Rhodospirillum rubrum. Arch. Mikrobiol. 66, 348–364 (1969).
Sojka, G. A., Gest, H.: Integration of energy conversion and biosynthesis in the photosynthetic bacterium Rhodopseudomonas capsulata. Proc. nat. Acad. Sci. (Wash.) 61, 1468–1493 (1968).
Sojka, G. A., Gest, H.: Effects of exogenous adenosine triphosphate on growing cells of Rhodopseudomonas capsulata. Bact. Proc. 64 (1970).
—, Baccarini, A., Gest, H.: Energy flux and membrane synthesis in photosynthetic bacteria. Science 166, 113–115 (1969).
—, Din, G. A., Gest H.: Integration of energy conversion and biosynthetic processes in bacterial photosynthesis. Nature (Lond.) 216, 1021–1022 (1967).
Strehler, B. L., Totter, J. R.: Firefly luminescence in the study of energy transfer mechanisms: I. Substrate and enzyme determination. Arch. Biochem. 40, 28–41 (1952).
Welsch, F., Smith, L.: Kinetics of synthesis and utilization of adenosine triphosphate by intact cells of Rhodospirillum rubrum. Biochemistry 8, 3403–3408 (1969).
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Schmidt, G.L., Kamen, M.D. Control of chlorophyll synthesis in Chromatium vinosum . Archiv. Mikrobiol. 76, 51–64 (1970). https://doi.org/10.1007/BF00409314
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DOI: https://doi.org/10.1007/BF00409314