Skip to main content
Log in

Protein turnover in a psychrotrophic bacterium

  • Original Papers
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Protein breakdown in pulse-labelled and longlabelled cells of Arthrobacter S 1-55, a psychrotrophic bacterium, has been assessed at different temperatures. The temperature at which the cells were grown and labelled affected the breakdown of pulsed-labelled but not long-labelled proteins. Inhibitors of ATP synthesis inhibited proteolysis. Miscoding antibiotics stimulated the production of rapidly degradable proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Amelunxen RE, Murdoch AL (1983) Microbial life at high temperatures. Mechanisms and molecular aspects. In: Kushner DJ (ed) Microbial life in extreme environments. Academic Press, New York, pp 217–270

    Google Scholar 

  • Bubella B, Holdsworth ES (1966) Amino acid uptake, protein and nucleic acid synthesis and turnover in Bacillus stearothermophilus. Biochim Biophys Acta 123:364–375

    PubMed  Google Scholar 

  • Carrier MJ, Kogut M, Hipkiss AR (1980) Relationship between protein breakdown and growth inhibition induced by aminoglycoside antibiotics and the effect of chloramphenicol and rifampicin. FEMS Microbiol Letts 9:161–165

    Article  Google Scholar 

  • Cashel M (1975) Regulation of bacterial ppGpp and pppGpp. Annu Rev Microbiol 29:310–318

    Article  Google Scholar 

  • Dice JF, Goldberg AL (1975) Relationship between in vivo degradative rates and isoelectric points of proteins. Proc Natl Acad Sci USA 72:3893–3897

    PubMed  Google Scholar 

  • Epstein I, Grossowicz N (1969) Intracellular protein breakdown in a thermophile. J Bacteriol 99:418–421

    PubMed  Google Scholar 

  • Goldberg AL, Dice JF (1974) Intracellular protein degradation in mammalian and bacterial cells (1). Annu Rev Biochem 43:835–869

    PubMed  Google Scholar 

  • Goldberg AL, St. John AC (1976) Intracellular protein degradation in mammalian and bacterial cells (2). Annu Rev Biochem 45:747–803

    Article  PubMed  Google Scholar 

  • Gounot AM (1976) Effects of temperature on the growth of psychrophilic bacteria from glaciers. Can J Microbiol 22:839–846

    PubMed  Google Scholar 

  • Gounot AM, Novitsky TJ, Kushner DJ (1977) Effects of temperature on the macromolecular composition and fine structure of psychrophilic Arthrobacter species. Can J Microbiol 23:357–362

    PubMed  Google Scholar 

  • Hershko A, Ciechanover A (1982) Mechanisms of intracellular protein breakdown. Annu Rev Biochem 51:335–364

    Article  PubMed  Google Scholar 

  • Hipkiss AR, Armstrong DW, Kushner DJ (1980) Protein turnover in a moderately halophilic bacterium. Can J Microbiol 26:196–203

    PubMed  Google Scholar 

  • Kemshead JT, Hipkiss AR (1974) Degradation of abnormal proteins in Escherichia coli: relative susceptibility of canavanyl proteins and puromycin peptides to proteolysis in vitro. Eur J Biochem 45:535–540

    PubMed  Google Scholar 

  • Kushner DJ (1978) Life in high salt and solute concentrations: Halophilic bacteria. In: Kushner DJ (ed) Microbial life in extreme environments. Academic Press, New York, pp 318–368

    Google Scholar 

  • Lin S, Zabin I (1972) β-Galactosidase: Rates of synthesis and degradation of incomplete chains. J Biol Chem 247:2205–2212

    PubMed  Google Scholar 

  • Nath K, Koch AL (1970) Protein degradation in Escherichia coli I measurement of rapidly and slowly decaying components. J Biol Chem 245:2889–2900

    PubMed  Google Scholar 

  • Olden K, Goldberg AL (1978) Studies on the eneergy requirement of intracellular protein degradation in Escherichia coli. Biochim Biophys Acta 542:385–398

    Google Scholar 

  • Pfueller SL, Elliot WH (1969) The extracellular α-amylase of Bacillus stearothermophilus. J Biol Chem 244:48–54

    PubMed  Google Scholar 

  • Pine MJ (1972) Turnover of intracellular proteins. Annu Rev Microbiol 26:103–126

    Article  PubMed  Google Scholar 

  • Pochon J, Tardieux P (1962) Techniques d'analyse en microbiologie du sol. Edition de la Tourelle, St. Mandé, France

    Google Scholar 

  • Rupley JA (1967) Susceptibility to attack by proteolytic encymes. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol XI, Enzyme structure: Hirs CHW (ed). Academic Press, New York, pp 905–917

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Potier, P., Hipkiss, A.R. & Kushner, D.J. Protein turnover in a psychrotrophic bacterium. Arch. Microbiol. 142, 28–33 (1985). https://doi.org/10.1007/BF00409232

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00409232

Key words

Navigation