Abstract
The extracellular polysaccharides (EPS) of six strains of cowpea rhizobia were examined. The strains (MI50A, M6-7B, IRC253) produced polysaccharides containing glucose, galactose and mannose in a molar ratio of 2:1.1:1, 1:1.3:3.1 and 1:1.3:3.5 respectively. Two strains (513-B and Ez-Aesch) produced polysaccharides containing galactose and mannose in a molar ratio of 2:3. Mannose was the only sugar detected in the EPS of strain IRC291. Pyruvate, acetate, glucuronic acid and galacturonic acid were not detected in any strain.
Similar content being viewed by others
Abbreviations
- EPS:
-
Extracellular polysaccharide
- YEMA:
-
yeast-extract mannitol agar
- YEMB:
-
yeast extract mannitol broth
References
Ahmad MH, Eaglesham ARJ, Hassouna S (1981a) Examining serological diversity of cowpea rhizobia by the ELISA technique. Arch Microbiol 130:281–287
Ahmad MH, Eaglesham ARJ, Hassouna S, Seaman B, Ayanaba A, Mulongoy A, Pulver EL (1981b) Examining the potential for inoculant use with cowpeas in West African soils. Trop Agric (Trin) 58:325–335
Ahmad MH, Uddin R, McLaughlin W (1984) Characterization of rhizobia from wild legumes. FEMS Microbiol Lett 24:197–204
Albersheim P, Nevins DJ, English, PD, Karr A (1967) A method for the analysis of sugars in cell-wall polysaccharides by gas liquid chromatography. Carbohyd Res 5:340–345
Allen EK, Allen O (1981) The Leguminosae. University of Wisconsin Press, Madison
Dudman WF (1976) The extracellular polysaccharides of Rhizobium japonicum: Compositional studies. Carbohyd Res 46:97–110
Dudman WF (1978) Structural studies of the extracellular polysaccharides of Rhizobium japonicum strains 71A, CC708 and CB 1793. Carbohyd Res 66:9–23
Fred ED, Baldwin IR, McCoy E (1932) Root nodule bacteria and Leguminous bacteria. University of Wisconsin Press, Madison
Ghai SK, Hisamatsu M, Amenura A, Harad T (1981) Production and chemical composition of extracellular polysaccharides of Rhizobium. J Gen Microbiol 122:33–40
Garegg PJ, Lindberg B, Onn T, Holme T (1971) Structural studies on the two mucoid mutants of Salmonella typhimurium. Acta Chem Scand 25:1185–1194
Guinn G (1967) An ultrasensitive chemical test for quantitative chromatography of sugars. J Chromatog 30:178–182
Hestrin S (1949) The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine and its analytical applications. J Biol Chem 18:249–261
Hough L, Jones JKN, Wodman WH (1950) Improved methods for the separation and detection of the sugars and their methylated derivatives on the paper chromatogram. J Chem Soc 2:1702–1706
Kennedy LD, Bailey RW (1976) Monomethyl sugars in extracellular polysaccharides from slow-growing Rhizobia. Carbohyd Res 49:451–454
Lowry O, Rosebrough M, Farr A, Randall R (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
McLaughlin W, Ahmad MH (1984) Intrinsic antibiotic resistance and streptomycin uptake in cowpea rhizobia. FEMS Microbiol Lett 21:299–303
Mort AJ, Bauer WD (1980) Composition of the capsular and extracellular polysaccharides of Rhizobium japonicum. Plant Physiol 66:158–163
Partridge SM (1949) Aniline Phthalate as a spray reagent for chromatography of sugars. Nature (Lond) 164:433–444
Seifter S, Dayton S, Nonic B, Muntwyler E (1950) The estimation of glycogen with anthrone reagent. Arch Biochem 25:191–200
Somme R (1974) Chemical analysis of exocellular, acid polysaccharides from seven Rhizobium strains. Carbohyd Res 33:89–96
Trinick M (1973) Symbiosis between Rhizobium and the nonlegume, Trema aspera. Nature (Lond) 244:459–460
Vincent JM (1970) A manual for the practical study of root nodule bacteria. IBP Handbook No. 15. Blackwell Scientific Publication, Oxford
Zevenhuizen LPTM (1973) Methylation analysis of acidic expolysaccharides of Rhizobium and Agrobacterium. Carbohyd Res 26:409–419
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hollingsworth, R., Smith, E. & Ahmad, M.H. Chemical composition of extracellular polysaccharides of cowpea rhizobia. Arch. Microbiol. 142, 18–20 (1985). https://doi.org/10.1007/BF00409230
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00409230