Summary
Cell-free extracts of methane-utilizing bacteria (methylobacteria) were examined for the presence of enzymes of the tricarboxylic acid cycle. Representative organisms of the Type II group had a complete set of enzymes for the operation of this cycle. Members of the Type I group lacked α-ketoglutarate dehydrogenase. All the methylobacteria examined had an NADH-oxidase which had the properties of a flavoprotein type enzyme. Glucose-6-phosphate dehydrogenase and gluconate-6-phosphate dehydrogenase was NADP-specific and could only be detected in cell extracts of Type I methylobacteria.
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References
Amarisingham, C. R., Davis, B. D.: Regulation of a α-ketoglutarate dehydrogenase formation in Escherichia coli. J. biol. Chem. 240, 3664–3668 (1965).
Arrigoni, O., Singer, T.P.: Limitations of the phenazine methosulphate assay for succinate and related dehydrogenases. Nature (Lond.) 193, 1256–1258 (1962).
Axelrod, B., Jang, R.: Purification and properties of phosphoriboisomerase from alfalfa. J. biol. Chem. 209, 847–855 (1954).
Davies, S., Whittenbury, R.: Fine structure of methane and other hydrocarbonutilizing bacteria. J. gen. Microbiol. 61, 227–232 (1970).
Di Pietro, D. L., Weinhouse, S.: Hepatic glucokinase in the fed, fasted and alloxandiabetic rat. J. biol. Chem. 235, 2542–2545 (1960).
Haba, G. De La, Racker, E.: Crystalline transketolase from baker's yeast. In: Methods in enzymology, Vol. I, pp. 375–380. S. P. Colowick and N. O. Kaplan, ed, New York: Academic Press 1955.
Heptinstall, J., Quayle, J. R.: Pathways leading to and from serine during growth of Pseudomonas AMI on Cl compounds or succinate. Biochem. J. 117, 563–572 (1970).
Hoare, D. S., Taylor, B. F., Patel, R.: Obligate methylotrophs and obligate autotrophs. 10th International Congress for Microbiology, Mexico (1970).
Horecker, B. L., Smyrniotis, P. Z.: 6-phosphogluconic dehydrogenase. In: Methods in enzymology, Vol. I, pp. 323–327. S. P. Colowick and N. O. Kaplan, ed. New York: Academic Press 1955.
Kemp, M. B., Quayle, J. R.: Microbial growth on Cl compounds. Incorporation of Cl units into allulose phosphate by extracts of Pseudomonas methanica. Biochem. J. 99, 41–48 (1966).
Kemp, M. B., Quayle, J. R.: Microbial growth on Cl compounds; uptake of (14C)-formaldehyde and (14C)formate by methane grown Pseudomonas methanica and determination of the hexose labelling patterns after brief incubation with (14C)-methanol. Biochem. J. 102, 94–102 (1967).
Kornberg, A.: Isocitric dehydrogenase of yeast (TPN). In: Methods in enzymology, vol. I, pp. 705–707. S. P. Colowick and N. O. Kaplan, ed. New York: Academic Press 1955.
Kornberg, A., Horecker, B. L.: Glucose-6-phosphate dehydrogenase. In: Methods in enzymology, Vol. I, pp. 323–327. S. P. Colowick and N. O. Kaplan, ed. New York: Academic Press 1955.
Lawrence, A. J., Quayle, J. R.: Alternative carbon assimilation pathways in methane-utilizing bacteria. J. gen. Microbiol. 63, 371–374 (1970).
Lowry, O. H., Rosebrough, N. J., Farr, A. J., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).
Mehler, A. H., Kornberg, A., Grisolia, S., Ochoa, S.: The enzymatic mechanism of oxidation reductions between malate or isocitrate and pyruvate. J. biol. Chem. 174, 961–977 (1948).
Ochoa, S.: Biosynthesis of tricarboxylic acids by carbon dioxide fixation. J. biol. Chem. 174, 133–157 (1948).
Patel, R., Hoare, D. S., Taylor, B. F.: Biochemical basis for the obligate C-1 dependence of Methylococcus capsulatus. Bact. Proc. 69, 128 (1969).
Racker, E.: Spectrophotometric measurements of the enzymatic formation of fumaric and cis-aconitic acids. Biochim. biophys. Acta (Amst.) 4, 211–214 (1950).
Ribbons, D. W., Harrison, J. E., Wadzinski, A. M.: Metabolism of single carbon compounds. Ann. Rev. Microbiol. 24, 135–158 (1970).
Rutter, W. J., Hunsley, J. R.: Fructose diphosphate aldolase. I. Yeast. In: Methods in enzymology, Vol. IX, pp. 480–486. In: S. P. Colowick and N. O. Kaplan, ed. New York: Academic Press 1966.
Smith, A. J., London, J., Stanier, R. Y.: Biochemical basis of obligate autotrophy in blue-green algae and Thiobacilli. J. Bact. 94, 972–983 (1967).
Sols, A., Salas, M. L.: Phosphofructokinase. III. Yeast. In: Methods in enzymology, Vol. IX, pp. 436–442. In: S. P. Colowick and N. O. Kaplan, ed. New York: Academic Press 1966.
Srere, P. A., Brazil, H., Gonen, L.: The citrate condensing enzyme of pigeon breast muscle and moth flight muscle. Acta chem. scand. 17, 129–134 (1963).
Whittenbury, R., Phillips, K. C., Wilkinson, J. F.: Enrichment, isolation and some properties of methane-utilizing bacteria. J. gen. Microbiol. 61, 205–218 (1970).
Wu, R., Racker, E.: Regulatory mechanisms in carbohydrate metabolism. III. Limiting factors in glycolysis of ascites tumour cells. J. biol. Chem. 234, 1029 to 1041 (1959).
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Davey, J.F., Whittenbury, R. & Wilkinson, J.F. The distribution in the methylobacteria of some key enzymes concerned with intermediary metabolism. Archiv. Mikrobiol. 87, 359–366 (1972). https://doi.org/10.1007/BF00409135
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DOI: https://doi.org/10.1007/BF00409135