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Glyceraldehyde 3-phosphate dehydrogenase of Scenedesmus obliquus the effect of ATP on the dithiothreitol-promoted induction of NADPH-dependent activity

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Abstract

Incubation of hexadecameric glyceraldehyde 3-phosphate dehydrogenase of Scenedesmus obliquus with dithiothreitol caused a transient increase in NADPH-dependent activity. When ATP was also included in the incubation, the induced NADPH-dependent activity was stabilised. The rate of induction of NADPH-dependent activity increased hyperbolically with respect to ATP concentration. The effect of binding of ATP to the enzyme was two-fold; not only did it stimulate the dithiothreitol-promoted formation of an enzyme species with high NADPH-dependent activity it also prevented the inactivation of this species. The high concentration of ATP required for the stimulation of the NADPH-dependent activity compared with those for NADPH suggest that activation by ATP is unlikely to be of physiological significance.

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  1. Department of Biochemistry, University of Liverpool, P.O. Box, 147, L69 3BX, Liverpool, UK

    Sandra Woodrow & Roy Powls

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  1. Sandra Woodrow
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  2. Roy Powls
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Woodrow, S., Powls, R. Glyceraldehyde 3-phosphate dehydrogenase of Scenedesmus obliquus the effect of ATP on the dithiothreitol-promoted induction of NADPH-dependent activity. Arch. Microbiol. 120, 177–180 (1979). https://doi.org/10.1007/BF00409105

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  • DOI: https://doi.org/10.1007/BF00409105

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