Summary
The activity of NADH oxidase in cell fractions of R. rubrum was measured. In cells grown photosynthetically (anaerobic in the light) and semiaerobically in the dark, the highest activity was found to be in the 19,000xg pellet. This consisted preponderantly of cytoplasmic membrane and cell wall material. Bchl was more enriched in the purified thylakoids than the NADH oxidase activity.
In extracts of cells grown aerobically the oxidase activity was higher than in cells grown anaerobically or semiaerobically. The highest activity was recovered in the 220,000xg pellet. The data suggest that NADH oxidase as well as bacteri ochlorophyll can be localized in the total intracellular membrane system. However, the distribution is inhomogeneous. Most of the NADH oxidase activity is localized in the c. m. region and most of bchl in the thylakoids.
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Abbreviations
- c. m.:
-
cytoplasmic membrane
- th.:
-
Thylakoid
- bchl:
-
bacteriochlorophyll
- Tris:
-
Tris (hydroxymethyl) amino-methane
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Throm, E., Oelze, J. & Drews, G. The distribution of NADH oxidase in the membrane system of Rhodospirillum rubrum . Archiv. Mikrobiol. 72, 361–370 (1970). https://doi.org/10.1007/BF00409035
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DOI: https://doi.org/10.1007/BF00409035