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Metabolic studies with neurospora: Action of 5-bromo- and 5-nitrouracils

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Summary

There is no growth inhibition of Neurospora crassa even in presence of high levels of 5-bromouracil. Although there is no significant change in the DNA and RNA contents, the analogue is utilized by the organism, a part of it being incorporated in DNA. There is an increase in total folate and citrovorum factor activities with 5-bromouracil.

5-Nitrouracil causes a significant growth inhibition at higher concentration which is accompanied by a decrease in pteroylglutamic acid and citrovorum factor levels in the organism.

Pteroylglutamic acid and thymidine cause a partial reversal of growth inhibition by 5-nitrouracil and complete reversal when the two metabolites are added simultaneously.

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Authors and Affiliations

  1. Department of Chemical Technology, University of Bombay, India

    M. R. Vora & D. V. Tamhane

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  1. M. R. Vora
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  2. D. V. Tamhane
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Vora, M.R., Tamhane, D.V. Metabolic studies with neurospora: Action of 5-bromo- and 5-nitrouracils. Archiv. Mikrobiol. 54, 237–245 (1966). https://doi.org/10.1007/BF00408996

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  • DOI: https://doi.org/10.1007/BF00408996

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