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Die Biosynthese der Poly-β-hydroxybuttersäure durch Knallgasbakterien

III. Synthese aus Kohlendioxyd

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Zysammenfassung

Bereits nach 8 sec 14CO2-Fixierung ist die aus Hydrogenomonas H 16 isolierte Poly-β-hydroxybuttersäure (PHBS) gleichmäßig radioaktiv markiert.

Es werden Beweise dafür erbracht, daß die PHBS aus Kohlendioxyd über 3-Phosphoglycerinsäure, Brenztraubensäure, Acetyl-CoA und Acetacetyl-CoA synthetisiert wird.

Während der PHBS-Synthese geht so eines von drei fixierten CO2-Molekülen durch oxydative Decarboxylierung der Brenztraubensäure wieder verloren. Damit steht im Einklang, daß die CO2-Fixierungsleistung wachsender Zellen größer ist als die PHBS-speichernder.

Nur ein Zehntel der Ribulose-1,5-diphosphat-Carboxylase, die für die gemessene autotrophe CO2-Fixierungskapazität erforderlich wäre, konnte im Rohextrakt von Hydrogenomonas H 16 nachgewiesen werden. Das Enzym ließ sich 20 fach anreichern.

Summary

Poly-β-hydroxybutyric acid (PHBA) isolated from Hydrogenomonas H 16 following an 8 sec 14CO2-incorporation is already uniformly labelled.

It was shown, that the synthesis of PHBA from carbon dioxide takes place via 3-phosphoglyceric acid, pyruvic acid, acetyl-CoA and acetoacetyl-CoA.

During the synthesis of PHBA, one of three CO2-molecules previously fixed is lost in an oxydative decarboxylation of pyruvic acid. It is therefore evident that the CO2-fixation of growing cells will be larger than that of cells storing PHBA.

Only one tenth of the ribulose-1,5-diphosphate carboxylase, which would be necessary for the measured CO2-fixation, could be determined in the crude extract of Hydrogenomonas H 16. The carboxylase was purified about 20-fold.

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Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Göttingen, Göttingen, Germany

    Gerhard Gottschalk

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Auszug aus der gleichlautenden Dissertation der mathematisch-naturwissenschaftlichen Fakultät der Universität Göttingen 1963.

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Gottschalk, G. Die Biosynthese der Poly-β-hydroxybuttersäure durch Knallgasbakterien. Archiv. Mikrobiol. 47, 236–250 (1964). https://doi.org/10.1007/BF00408941

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  • DOI: https://doi.org/10.1007/BF00408941

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