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Bakterielles Wachstum bei geringen Substratkonzentrationen

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Zusammenfassung

Die theoretische Beziehung zwischen Populationsdichte, Wachstumsrate und der Konzentration des wachstumsbegrenzenden Substrates (Lactat) wurde in Chemostat-Kulturen von Spirillum serpens und Spirillum spec. überprüft. Sobald die Konzentration des begrenzenden Substrates im zulaufenden Medium einen bestimmten Wert unterschritt, wurde die Population ausgewaschen. Diese Schwellenkonzentration des Substrates ist an die Gegenwart suboptimaler Wachstumsbedingungen gebunden, die durch die Stoffwechselaktivität der Organismen sekundär verbessert werden. Unterhalb eines solchen Schwellenwertes der Substratkonzentration-und damit der Populationsdichte-reicht die Produktion eines hypothetischen Metaboliten nicht mehr aus, um eine Wachstumsförderung aufrechtzuerhalten. Die Schwellenkonzentration des Substrates nimmt dementsprechend mit steigender Wachstumsrate ab. Sie kann weiterhin durch Herabsetzen des Redoxpotentials mit Hilfe eines Zusatzes von Ascorbinsäure verringert-aber nicht eliminiert-werden. Das bedeutet, daß ein Teil der wachstumsfördernden Aktivität auf die Reduktionskraft der Population zurückzuführen ist.

Summary

Population density and growth rate of cultures of Spirillum serpens and Spirillum sp. were checked as functions of the concentration of the limiting substrate (lactate) in the chemostat. It was found that under suboptimal conditions of growth, cells may stimulate the rate of growth by metabolic activity. This internal factor becomes perceptible only when the concentration of the limiting substrate (and as a consequence, the population density) drops below a certain value at which the production of the proposed metabolite does not meet the demand for optimal growth-conditions. Threshold concentrations of the limiting substrate-below which washout of the population occurred at a fixed dilution rate-could be decreased (but not eliminated) by lowering the redox potential of the medium by the addition of ascorbic acid. Thus, in the case studied, part of the growth stimulating action of the population was attributed to the reducing power of the cells. It is effective only in the presence of a suboptimal redox potential and increases with the growth rate.

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Authors and Affiliations

  1. Institut für Mikrobiologie der Universität Göttingen, Göttingen, Deutschland

    Holger W. Jannasch

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  1. Holger W. Jannasch
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Gekürzte Fassung der gleichlautenden Habilitationsschrift der mathematischnaturwissenschaftlichen Fakultät der Universität Göttingen 1963.

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Jannasch, H.W. Bakterielles Wachstum bei geringen Substratkonzentrationen. Archiv. Mikrobiol. 45, 323–342 (1963). https://doi.org/10.1007/BF00408931

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  • DOI: https://doi.org/10.1007/BF00408931

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