Summary
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1.
14C-labeled substances, derived from cultures of Blakeslea trisporaplus, are converted to radioactive trisporic acids (TA) by B. trispora-minus. In the same manner, partial conversion of 14C-labeled substances, isolated from minus cultures, to 14C-labeled TA by a plus culture, is demonstrated.
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2.
The synthesis of these TA precursors is stimulated by TA itself in plus as well as in minus mycelia. This stimulating effect of TA is inhibited by 5-fluorouracil.
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3.
The high rate of TA synthesis in a mated culture of B. trispora can be explained by these results: TA exerts a strong promoting action upon its own synthesis.
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Abbreviations
- TA:
-
Trisporic acids B+C
- P+:
-
neutral substances, produced by plus cultures, extractable with hexane, with R F=0.55 on thin-layer chromatograms (see Materials and Methods)
- P-:
-
neutral substances, produced by minus cultures, extractable with hexane (R F=0.05–0.13 and 0.3–0.5), and diethyl ether (R F=0.3–0.5)
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Werkman, T.A., van den Ende, H. Trisporic acid synthesis in Blakeslea trispora . Archiv. Mikrobiol. 90, 365–374 (1973). https://doi.org/10.1007/BF00408930
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DOI: https://doi.org/10.1007/BF00408930